摘要
在NH3-NH4Cl缓冲溶液介质中,痕量Cu2+对H2O2氧化考马斯亮蓝G250(CBBG)褪色反应的显著催化作用,建立了催化动力学光度法测定痕量铜的新方法。线性范围为0.02～0.20μg/mL,检出限为1.34×10-9g/mL。考察了20多种共存离子的影响。方法用于发样中痕量铜离子的测定,相对标准偏差小于3.4%,加标回收率为97%～105%。
Copper（Ⅱ） catalyze the oxidation of coomassie brilliant blue G250（CBBG）by hydrogen peroxide in NH3-NH4Cl buffer solution and copper（Ⅱ） can be determined by spectrophometry with the decolorization effete.Therefore,a new sensitive catalytic kinetics spectrophotometric method is proposed.The range of determination for copper（II） is 0.02～0.20μg/mL.The detection limit of the method is 1.34×10-9g/mL.The interferences of foreign ions are also studied.The procedure has been used to determine copper（Ⅱ） in human hair,with the relative standard deviation of below 3.4% and the average recovery of 97%～105%.
出处
《分析试验室》
CAS
CSCD
北大核心
2012年第8期83-85,共3页
Chinese Journal of Analysis Laboratory
关键词
催化动力学
铜
过氧化氢
考马斯亮蓝G250
Catalytic kinetic analysis； Copper（Ⅱ）； Hydrogen peroxide； Coomassie brilliant blue G250
