摘要
本文合成了偶氮类试剂3,5-二甲酸-4'-羟基偶氮苯,并研究了其与铜(Ⅱ)的显色反应。应用分光光度计测定了3,5-二甲酸-4'-羟基偶氮苯的用量、四硼酸钠缓冲溶液用量对生成配合物的影响,建立了一种测定口蘑中微量铜的新方法。结果表明:铜(Ⅱ)的质量浓度在0~2.5μg·mL^-1内遵从比尔定律,其回归方程为Y=0.1696x+0.0237,R^2=0.9921,摩尔吸光系数为4.3×104 L·mol^-1·cm^-1,铜(Ⅱ)与3,5-二甲酸-4'-羟基偶氮苯形成1∶1的配合物。加标回收率为97.0%~101.3%,RSD为0.93%~1.50%。本实验采用新的紫外分光光度法检测铜离子,操作简单、选择性好,用于口蘑中铜的测定,结果为(5.95±0.49)mg/100 g,与ICP光谱仪测得量为(5.89±0.09)mg·100 g^-1几乎一致。
The azo-based chromogenic reagent 3,5-dicarboxylic acid-4'-hydroxyazobenzene was synthesized and its color reaction with copper(II)was also investigated.The amount of 3,5-dicarboxylic acid-4'-hydroxyazobenzene and the effect of sodium tetraborate buffer solution on the formation of complexes were determined by spectrophotometer.A new measured method for the micro copper(II)in tricholoma was established.The results showed that the mass concentration of copper(II)obeyed Bill’s law in the range of 0~2.5μg·mL^-1.The regression equation was Y=0.1696x+0.0237,R^2=0.9921,and the molar absorptivity was 4.3×104 L·mol^-1·cm^-1.Copper(Ⅱ)complexes with 3,5-dicarboxylic acid-4'-hydroxyazobenzene formed a 1∶1 complex.The recovery of standard addition was 97.0%~101.3%and RSD was 0.93%~1.50%.A new ultraviolet spectrophotometric method for the determination of copper ion in Tricholoma was developed.The method was simple and selective.The result was(5.95±0.49)mg·100 g^-1,which was almost the same as that measured by ICP spectrometer(5.89±0.09)mg·100 g^-1.
作者
苏逸菲
汤梦怡
徐善东
李强
SU Yi-fei;TANG Meng-yi;XU Shan-dong;LI Qiang(College of Biological Sciences and Technology,Beijing Forestry University,Beijing 100083,China;College of Science,Beijing Forestry University,Beijing 100083,China)
出处
《食品工业科技》
CAS
北大核心
2019年第17期231-237,共7页
Science and Technology of Food Industry
基金
中央高校基本科研业务费专项资金资助(2015ZCQ-LY-03)
国家自然科学基金资助项目(21671021)
关键词
偶氮化合物
紫外分光光度法
铜
口蘑
azoic compound
ultraviolet spectrophotometry
copper
Tricholoma
