摘要
目的探讨细胞外超氧化物歧化酶(EC-SOD)和谷胱甘肽硫转移酶M1(GSTM1)基因多态性及吸烟与口腔癌风险之间的关系。方法利用病例-对照分子流行病学研究方法和聚合酶链反应技术对600例口腔癌患者和600健康正常对照者DNA进行EC-SOD和GSTM1基因分型,logistic回归方法估计基因、基因与吸烟的交互作用对口腔癌发病的危险度。结果 EC-SOD各基因型口腔癌病例组与对照组间的频率分别为在C/C型61.17%、78.50%,C/G型38.33%、21.50%,分布差异有显著性(P<0.01),EC-SOD(C/G)基因型使口腔癌的发生的危险性增加(OR=2.27,95%CI 1.73-4.02)。口腔癌组GSTM1(-)基因型频率(69.17%)显著高于对照组(44.17%)(P<0.01),GSTM1(-)基因型增加了口腔癌发生的危险性(OR=2.84,95%CI 1.95-4.47).基因突变的协同分析发现EC-SOD(C/G)/GSTM1(-)在口腔癌组和对照组中的分布频率分别为30.50%和6.67%,分布有显著性差异(P<0.01)。EC-SOD(C/G)/GSTM1(-)患口腔癌的风险性明显高于EC-SOD(C/C)/GSTM1(+)基因型者(OR=8.16,95%CI 3.73-12.91)。病例组的吸烟率显著高于对照组的吸烟率(P<0.01),吸烟显著提高口腔癌发生的危险性(OR=2.66,95%CI 1.45-4.36)。EC-SOD(C/G)及GSTM1(-)与吸烟有协同作用(OR=25.11,95%CI 12.37-36.62)。结论 EC-SOD(C/G)和GSTM1(-)是口腔癌的易患因素,二者对口腔癌的发生有协同作用,吸烟与口腔癌的易感性也有关,EC-SOD(C/G)和GSTM1(-)与吸烟在口腔癌的发生上具有相互促进作用。
Objective To investigate the correlation of the polymorphisms of EC-SOD and GSTM1, as well as smoking, with oral cancer risk. Methods A case-control study and polymerase chain reaction （PCR） technique was used to identify the genotype of EC-SOD and GSTM1 in 600 oral cancer cases and 600 controls. Logistic mg, ression was conducted to evaluate ＇the odds ratio of relative gene and the interaction between the genes and smoking with oral cancer. Results The frequencies of EC-SOD genotype in casesand controls were 61.17% and 78.50% for C/C, and 38.33% and 21.50% for C/G respectively （P 〈 0.01 ）. EC-SOD （C/G） correlated with susceptible to oral cancer （0R=2.27, 95% CI 1.73 -4.02）. The frequency distribution of GSTM1 （ - ） was significantly different in patients （69. 17% ） and controls （44. 17% ） （P 〈0. 01）. GSTM1 （ - ） increased the risk of developing oral cancer （ OR = 2.84,95% CI 1.95 - 4. 47 ）. Combined polymorphism analysis showed that the percentage of EC-SOD （C/G）/GSTM1 （ - ） for oral cancer and control groups was 30. 50% and 6.67% respectively） （P 〈0. 01 ）. The risk of developing oral cancer in those with EC-SOD（C/G）/GSTM1 （ - ） genotype was significantly higher than that with EC-SOD （C/C）/GSTM1 （ ＋ ） genotype （ OR = 8.16, 95% CI 3.73 - 12.91）. There were significant differences in smoking rate between the two groups（P 〈 0. 01 ）, smoking correlated with susceptible to oral cancer（ OR = 2. 66,95% CI 1.45-4. 36）, and statistical analysis suggested an interaction between smoking and EC- SOD（C/G）/GSTM1 （ - ） genotype, which increase the risk of oral cancer（OR = 25.11, 95% CI 12.37 -36. 62）. Conclusion EC-SOD（C/G） and GSTM1 （ - ） are risk factors in oral cancer. Smoking is also related to the susceptibility to oral cancer. There may be a synergetic interaction among EC-SOD（C/G）, GSTM1 （-） and smoking on the elevated susceptibility of oral cancer.
出处
《卫生研究》
CAS
CSCD
北大核心
2012年第4期555-561,共7页
Journal of Hygiene Research
