摘要
目的通过观察通降解毒方药物血清对胃癌MKN-45细胞增殖的影响,以期佐证其临床抑制肿瘤生长的作用。方法制备通降解毒方药物血清,MKN-45细胞随机分为空白对照组、空白血清组、通降解毒方血清组、5-Aza-CdR组,培养24、48、72 h后观察胃癌细胞的形态变化,CCK8法测定胃癌细胞活性。结果通降解毒方血清组、5-Aza-CdR组均能抑制MKN-45细胞的增殖,与空白对照组比较,差异有统计学意义。结论通降解毒方药物血清能抑制体外胃癌细胞的增殖,从体外实验的角度可以解释其临床抗肿瘤的效果。
Objective ： To observe the effects of serum containing Tongjiangjiedu Decoction on proliferation of gastric cancer cell MKN--45 and to study its mechanism of anticancer in clinical. Methods：After Tongjiangjiedu Decoction-containing serum being prepared,MKN-45 cells were divided into the blank control group,the blank serum group,the Tongiiangjiedu Decoction-containing serum group and the 5-Aza-CdR group. Morphological changes observed after gastric cancer cells in culture 24,45,72h. Gastric cell viability was determined by CCK8. Results：Cell proliferation was obviously inhibited in the Tongjtangjiedu Decoction-containing serum group and the 5-Aza-CdR group. There were significant differences among above groups and the blank control group. Con- clusion：Serum containing Tonjgianjgiedu Decoction could suppress gastric cancer cell proliferation.Its clinical an- ti-tumor effect can be explained from the perspective of in vitro experiments.
出处
《中国中医急症》
2012年第8期1254-1254,1281,共2页
Journal of Emergency in Traditional Chinese Medicine
基金
贵州省科技厅联合基金资助(黔科合LS字[2011]010)
关键词
通降解毒方
胃癌细胞
细胞增殖
血清药理学
Tongjiangjiedu Decoction
Gastric cancer cell
Cell proliferation
Serum pharmacology
