摘要
研究了牛血清白蛋白(BSA)与全氟辛烷磺酸(PFOS)相互作用的共振光散射(RLS)光谱,建立了PFOS的共振光散射分析方法。在pH值为4.1的BR缓冲溶液中,全氟辛烷磺酸根阴离子与质子化的BSA通过静电引力和疏水作用形成离子缔合物,引起共振光散射强度(IRLS)显著增强,最大散射波长位于285.0 nm处,增强的散射信号强度与PFOS浓度在0.2～25.0μmol/L范围内呈线性关系,据此建立了测定PFOS的光散射分析方法,检出限为20.0 nmol/L。讨论了体系的最佳反应条件及外来物质的干扰,并探讨了反应机理。建立的共振光散射法用于环境水样中PFOS的测定,RSD≤4.4%。
The interaction of bovine serum albumin （BSA） with perfluorooctane sulfonate （PFOS） was investigated with resonance light scattering（RLS） spectroscopy. A RLS method for the determination of PFOS was established. In pH = 4. 1 Britton-Robinson buffer solution, perfluorooctane sulfonate anions reacted with the protonated BSA by electrostatic forces and hydrophobic interactions to form ion-association complexes and resulted in greatly enhanced resonance light scattering signals characterized by a peak at 285.0 nm. The RLS intensity of system was proportional to the content of PFOS in the range of 0. 2 - 25.0 μmol/L, and the limit of detection was 20. 0 nmol/L. The optimum reaction conditions and interference of foreign substances were investigated. This RLS method was applied to the determination of PFOS in environmental samples with RSD ≤4. 4%.
出处
《应用化学》
CAS
CSCD
北大核心
2012年第8期969-973,共5页
Chinese Journal of Applied Chemistry
基金
国家自然科学基金(20877063)
西南大学大型仪器设备开放基金资助项目
关键词
牛血清白蛋白
全氟辛烷磺酸
共振光散射
bovine serum albumin, perfluorooctane sulfonate, resonance light scattering
