摘要
目的 探讨转化生长因子-β3（TGF-β3）与牙髓干细胞联合应用能否显著促进兔面神经的损伤修复.方法 取16只成年健康新西兰大白兔,制备面神经上颊支7mm缺损并于损伤局部进行硅胶管套接的动物模型.右侧面神经随机设为（TGF-β3＋牙髓干细胞）实验组（8只兔）和TGF-β3对照组1（8只兔）,左侧面神经设为PBS液对照组2（8只兔）和正常对照组（8只兔）.术后3月,进行大体形态观察、组织学观察及电生理检测.结果实验兔16只均进入分析,实验组再生神经直径与近、远端神经干相当,无神经瘤形成,外膜血管丰富,质地坚韧.再生组织切片HE染色显示：实验组面神经外膜完整,神经纤维排列相对较整齐,形态较完整,髓鞘肿胀,仍可见肥大增生的雪旺细胞,胞核浓密,束间血管较多.光学显微镜图像分析显示：实验组再生神经纤维总数多于其他2个对照组,再生神经纤维直径也远远大于其他2个对照组,其差异具有统计学意义（p＜0.05）.超薄切片透射电镜结果显示：实验组的再生纤维以有髓神经纤维为主,形态规则,髓鞘板层结构清晰,轴浆内细胞器丰富.神经电生理检测结果显示实验组神经肌肉动作电位的潜伏期明显短于其他2个对照组[实验组（1.96±0.32） ms,对照组1（2.35±0.41） ms,对照组2（3.42±0.55）ms,p＜0.05],并且实验组神经肌肉动作电位的波幅明显高于其他2个对照组[实验组（11.06±3.25） mV,对照组1（8.40±1.68） mV,对照组2（4.62±0.77） mV,p＜0.05].结论TGF-β3与牙髓干细胞联合应用能显著提高面神经损伤后的修复作用.
Objective To investigate the effects of using transforming growth factor-β （TGF-β3） combined with dental pulp stem cells （DPSCs） on the regeneration of rabbit facial nerve. Methods Sixteen New Zealand adult rabbits were selected randomly. These rabbits were divided into three groups （TGF-β3 and DPSCs treated group, TGF-β3 control group and PBS control group, treatment group （silicone guidance channel with collagen protein sponge were filled with TGF-β3 and DPSCs）, TGF-133 control group （silicone guidance channel with collagen protein sponge were filled with TGF-β3） and PBS control group （silicone guidance channel with collagen protein sponge were filled with PBS）. After 3 months, a series of examinations were performed, including gross morphology, histological staining, neuroeiectrophysiological tset. Results In the experimental group, the diameter of the regenerating nerve and near distal nerve stem were almost the same. There were no formation of neuroma. The adventitial angiogenesis was rich and with tough texture. 3 months after operation, in the experimental group, the facial nerve membrane integrity, nerve fibers arranged in neat rows, form a more complete, myelin swelling. The total number of regeneration of nerve fibers in the experimental group were more than of control groups, statistical analysis was significant （P〈0.05）. Diameter of regenerating nerve fibers in the experimental group were greater than that of control groups, statistical analysis was significant （P〈0.05）. The ultra-thin section showed that the regenerated fibers in the treatment group were mainly myelinated never fiber. The layer structure of myelin sheath was clear, and there were rich organells axoplasma. The neuroelectrophysiological examinations revealed that the latency of nerve and muscle action conduction in the treatment group was shorter than that of the control groups, the treatment group： （1.96±0.32） ms, the TGF-β3 control group： （2.35±0.41） ms, the PBS control group：
出处
《国际生物医学工程杂志》
CAS
2012年第3期160-164,I0004,共6页
International Journal of Biomedical Engineering
基金
国家自然科学基金资助项目（30960423）
关键词
面神经损伤
神经再生
牙髓干细胞
TGF-Β3
Facial nerve injury
Regenerating nerve
Dental pulp stem cells
Transforming growth factor β3
