摘要
根据O型FMDV全基因组序列设计引物,通过PCR扩增得到目的基因,并对猪O型FMDV的强弱毒株基因进行克隆与序列分析。结果表明:扩增得到的O型口蹄疫病毒温度敏感株L、P1、P2、P3(3C、3D)区目的基因片段大小分别为603、2 208、1 464、639、1 410 bp,与预期相符。各区序列拼接后与野生型猪O型FMDV序列比对,两者同源性为88%。
In order to analyse the differences between wild type strain and temperature-sensitive mutation strain of foot-and-mouth disease virus （FMDV） type O in swine, five pairs of primer were designed according to complete genome sequences of the FMDV type O in the GenBank, and the gene of virulent and attenuated strain of FMDV type O in swine was cloned by PCR amplification and its sequence was analyzed. The results showed that the predicted fragments were obtained by RT-PCR from L, P1,P2 and P3（3C, 3D） area of temperature-sensitive strain of FMDV type O, with fragment size of 603 kb, 2 208 kb, 1 464 kb, 639 kb and 1 410 kb, respectively. After sequence assembly, nucleotide sequence alignment results showed that the homology between wild type strain and temperature-sensitive mutation strain of FMDV type O was 88%.
出处
《湖南农业科学》
2012年第7期24-26,共3页
Hunan Agricultural Sciences
关键词
口蹄疫病毒
基因
克隆
序列分析
foot-and-mouth disease virus
gene
cloning
sequence analysis
