摘要
将猪FMDV毒株(O/GD/MSH/86)分别适应牛肾细胞(Bovinekidney,MD-BK)、幼仓鼠肾细胞(Babyhamsterkidney,BHK-21)和猪肾细胞(Porcinekidney,PK-15),研究了FMDV在宿主细胞上的嗜性。结果发现,该毒株在MD-BK细胞上不产生细胞病变(CPE),从细胞液中也未检测到病毒,而在BHK-21和PK-15细胞上能够稳定产生CPE。可见,猪FMDVO/GD/MSH/86毒株不能在MD-BK细胞上增殖,而在BHK-21和PK-15细胞上则增殖较好。
Swine foot-and-mouth disease virus﹙FMDV﹚﹙O/GD/MSH/86﹚were adapted to different cells, MDBK, BHK-21 and PK-15, the host tropism of FMDV was studied. The results showed that no cytopathic effect﹙CPE﹚was detected in MDBK cells, and no virus was determined in cell sap, but the virus could induce stable CPE in BHK-21 and PK-15 cells. Therefore, the swine FMDV couldn’t multiply in MDBK cells, but it could reproduction well in BHK-21 and PK-15 cells.
出处
《甘肃农业大学学报》
CAS
CSCD
2005年第3期306-310,共5页
Journal of Gansu Agricultural University
基金
973项目(课题5:分子遗传衍化关系研究)
关键词
猪
口蹄疫病毒
细胞
嗜性
swine
foot-and-mouth disease virus
cell
tropism
