摘要
目的观察普拉梭菌（Faecalibacterium prausnitzii，Fp）对2，4，6三硝基苯磺酸诱导的大鼠结肠炎的治疗效果，并初步探讨其作用机制。方法60只Sprague-Dawley大鼠均分为健康对照组、结肠炎模型对照组、Fp预防治疗组、F户上清液预防治疗组、Fp治疗组和Fp上清液治疗组。评估各组大鼠的疾病活动指数（DAD、结肠组织学损伤、粪便短链脂肪酸丁酸的含量，检测外周血、脾内叉头状螺旋转录调节因子3（Foxp3）调节性T细胞（Treg）及血清促炎因子IL-17、IL-6的水平。所有数据采用单因素方差分析进行统计学处理。结果与结肠炎模型对照组比较，Fp预防治疗组、Fp上清液预防治疗组、F声治疗组和F户上清液治疗组的DAI评分显著降低，结肠组织学表现明显改善，且Fp预防治疗组优于F声治疗组，F声上清液预防治疗组优于F声上清液治疗组。丁酸浓度在F声预防治疗组为（3091．08士485．50）×10^-6mol／L，F声上清液预防治疗组为（1714．64±351．25）×10^-6mol／L，Fp治疗组为（2064．75±295．04）×10^-6mol／L，F声上清液治疗组为（1089．13±321．23）×10^-6mol／L，Fp预防治疗组与其他各组比较差异有统计学意义（F=49．796，P〈0．01）。Fp上清液预防治疗组外周血Foxp3＋Treg水平最高；F户预防治疗组、F户上清液预防治疗组脾细胞Foxp3＋Treg水平明显高于其他各组。F户预防治疗组、Fp上清液预防治疗组、Fp治疗组和F户上清液治疗组大鼠血清IL-17、IL-6浓度较结肠炎模型对照组均明显降低。结论F声对炎症性肠病模型大鼠的肠道炎性反应修复有一定的促进作用。其机制可能与分泌丁酸盐，上调外周血和脾内的Foxp3＋Treg水平，抑制促炎因子IL-17、IL-6分泌，重建Treg／Thl7平衡来减轻局部肠道的炎性反应。
Objective To explore the therapeutic effects and the mechanisms of Faecalibacteriurn prausnitzii （Fp） on trinitro-benzene-sulfonic acid-induced colitis. Methods Sixty Sprague-Dawley rats were divided into healthy control group, colitis model control group, Fp pretreated group, Fp supernatant pretreated group, Fp treated group and Fp supernatant treated group. Disease activity index （DAI）, histological injury of colonic tissue, the content of hutyrate in feces, forkhead box protein 3（Foxp3） regulatory T cells （Treg） in peripheral blood and spleen and thelevel of interleukin （IL）-17 and IL-6 in serum were evaluated. All the data were statistical analyzed by single factor analysis of variance. Results Compared with colitis model control group, DAI significantly lowered and histological injury obviously improved in Fp pretreated group, Fp supernatant pretreated group, Fp treated group and Fp supernatant treated group. The effects of Fp pretreated group were better than those of Fp treated group and Fp supernatant pretreated group were better than Fp supernatant treated group. The concentration of butyrate in Fp pretreated group, Fp supernatant pretreated group, Fp treated group and Fp supernatant treated group was （3091.08± 485.50）×10^-6 tool/L, （1714. 64±51. 25）×10^-6 tool/L, （2064. 75±295.04） ×10^-6 mot/L and （1089.13±321.23）× 10^-6 mol/L respectively, there was significant difference between Fp pretreated group and other groups （F=49. 796, P〈0.01）. The peripheral blood level of Foxp3＋ Treg in Fp supernatant pretreated group was highest. The spleen level of Foxp3＋ Treg in Fp pretreated group and Fp supernatant pretreated group were significantly higher than that of other groups. The serum level of IL-17 and IL-6 in Fp pretreated group, Fp supernatant pretreated group, Fp treated group and Fp supernatant treated group was significantly lower than that of colitis model group. Conclusions Fp plays a role in promoting the repair of intestinal inflammatory reaction
出处
《中华消化杂志》
CAS
CSCD
北大核心
2012年第7期459-465,共7页
Chinese Journal of Digestion
基金
国家自然科学基金（81170359）
