摘要
目的检测FOXP3+调节性T细胞(Treg)及白介素(IL)-10在弥漫性大B细胞淋巴瘤(DLBCL)组织中的表达情况,探讨其与各临床病理因素及预后的关系。方法采用免疫组化SP法,以FOXP3及IL-10为标志物,对69例DL-BCL组织及26例反应性增生的淋巴结和扁桃体进行染色,对阳性细胞分别进行绝对值计数,同时对69例DLBCL患者进行随访。结果 DLBCL中FOXP3+ Treg细胞数目及IL-10+细胞数目与对照组相比,差异具有统计学意义(P<0.01)。FOXP3+ Treg及IL-10+细胞数目在各临床病理因素间差异无统计学意义(P>0.05)。非GC-DLBCL间质中FOXP3+ Treg数目、GC-DLBCL间质中IL-10阳性细胞数目与预后具有相关性(P<0.05)。结论 FOXP3+ Treg与IL-10可作为DLBCL预后因素之一。
Objective To investigate the expression of FOXP3＋Treg and IL-10 in diffuse large B-cell lymphoma（DLBCL）and detecte its clinicopathological significance.Methods The absolute number and correlated to phenotypic and clinical parameters of FOXP3＋Treg and IL-10＋ cells was studied by immunohistochemical SP methods from 69 patients of DLBCL and 26 cases of normal tonsils and lymph nodes.ROC curves were used to determine prognostic cut-off values of FOXP3＋Treg and IL-10＋ cells density.Results The absolute number of FOXP3＋Treg cells and IL-10＋cells in DLBCL was significantly higher than that in normal tonsils and lymph nodes（P〈0.01）.The expression of FOXP3＋Treg and IL-10 had no relationship to gender,age,clinical stage and pathological subgroup（P〉0.05）.The expression of FOXP3＋Treg cells in non-GC DLBCL and IL-10＋ cells in GC-like DLBCL was correlation to prognosis（P〈0.05）.Conclusion The expression of FOXP3＋Treg and IL-10 cells serves as a prognostic indicator of DLBCL.
出处
《安徽医科大学学报》
CAS
北大核心
2011年第5期462-466,共5页
Acta Universitatis Medicinalis Anhui
