摘要
目的 对浙江省腹泻儿童进行隐孢子虫的病原学诊断及感染情况分析。方法 从浙江大学附属儿童医院收集腹泻儿童粪便５４８ 份，每份样本先用改良抗酸染色法检查卵囊，发现阳性或可疑标本，再用免疫荧光染色法进一步确诊。结果 两种染色方法结合诊断共发现５７ 例阳性病人，隐孢子虫感染率为１０－４％ ，男女之间感染率无显著性差异（χ２ ＝ ０－００７，Ｐ＞ ０－０５），其中１ 岁以下婴儿感染率为５－７ ％ ，１ ～１０ 岁儿童感染率为１７－５％ ，两者之间存在显著性差异（χ２ ＝ １９－６９ ，Ｐ＜ ０－０５）。结论 改良抗酸染色法与免疫荧光染色法结合诊断能达到隐孢子虫确诊目的，由感染情况分析可得浙江省腹泻儿童中有较高的隐孢子虫感染率，其中１ 岁以下儿童感染率较１ 岁以上儿童明显降低，可能与１ 岁以下婴儿多母乳喂养有关。
Aim To etiological diagnose and analyze of Cryptosporidium infection of diarrhea children in zhejiang province Method 548 fecal specimens were collected from diarrhea children in affiliated children hospital of Zhejiang College Each fecal specimen was smeared and screened by modified acid-fast staining method for oocyst;the positive and suspected specimens were identified by immunofluorescent staining method Result As a result,57 cases of infection were found by modified acid-fast staining and immunofluorescent staining method with a positive rate of 10 4%(57/548) Among the positive specimens 19 cases were under the age of l year,the positive rate was 5 7%(19/332),and 38 cases were between the age 2 and 10 with a positive rate of 17 5%(38/216) Conclusion The best result could be obtained when modified acid-fast staining method was first used and followed by immunofluorescent staining method The study showed that there were high Cryptosporidium infective rate in diarrhea children of Zhejiang province,but the positive rate under the age of 1 year was much lower than older children,this may be due to breast feeding
出处
《中国人兽共患病杂志》
CSCD
北大核心
2000年第1期42-44,F003,共4页
Chinese Journal of Zoonoses
关键词
隐孢子虫
病原
诊断
腹泻
儿童
Cryptosporidium
Etiological diagnosis
Diarrhea
