摘要
建立反相高效液相色谱法测定维格列汀的含量。采用Venusil MP C18色谱柱(250mm×4.6mm,5μm),流动相为磷酸盐缓冲溶液(称取20mmol·L^(-1)磷酸二氢钾,用85%浓磷酸调至pH3.0)-乙腈(88:12,V/V),检测波长为210nm,流速为1.0mL·min^(-1),柱温为25℃。维格列汀在5—500μg·mL^(-1)范围内与峰面积呈良好的线性关系(r=0.9999),维格列汀的平均回收率为102.0%,RSD为2.65%。方法准确、简便,适用于维格列汀的定量分析。
The content of vildagliptin was determined by RP-HPLC.The separation conditions were as follows：column was Venusil MP C18（250mm×4.6mm,5μm）,mobile phase was phosphate buffer（pH of the weighed 20mmol·L^-1 monopotassium phosphate was adjusted to 3.0 by 85% phosphoric acid）-acetonitrile（88：12,V/V）,detection wavelength was 210nm,flow rate was 1.0 mL·min^-1 and the column temperature was 25℃.The vildagliptin had the good linear relationship with peak area in the rang of 5-500μg·mL-1（r=0.9999）,and the average recovery was 102.0% with RSD of 2.65%.This method is reliable,accurate and suitable,and can be applied to quantitative analysis of vildagliptin.
出处
《光谱实验室》
CAS
CSCD
2012年第4期2555-2558,共4页
Chinese Journal of Spectroscopy Laboratory
