摘要
目的:观察黄蜀葵花总黄酮(TFA)对人脐静脉血管内皮细胞(HUVEC)凋亡及凋亡相关蛋白fas表达的影响。方法:体外培养HUVEC,加入TFA,按加入药物浓度的不同分为4组:TFA 0μg·ml-1组(对照组)、TFA 5μg·ml-1组、TFA 10μg·ml-1组、TFA 20μg·ml-1组,培育72 h后应用流式细胞术检测各组HUVEC凋亡率及fas蛋白的表达。结果:HUVEC凋亡率对照组为10.1%、TFA 5μg·ml-1组为7.2%、TFA 10μg·ml-1组为3.9%、TFA 20μg·ml-1组为8.5%。与对照组比较:各浓度TFA组HUVEC凋亡率差异有统计学意义(P<0.01),各浓度TFA组HUVEC fas表达率均下降,差异有统计学意义(分别P=0.000,P=0.000,P=0.028)。结论:TFA浓度在5～20μg·ml-1范围可抑制HUVEC凋亡,其机制与下调凋亡相关蛋白fas的表达有关。
Objective：To observe the effects of total flavone of Abelmoschus manihot（TFA）on apoptosis and apoptosis-related protein fas expression in human umbilical vein endothelial cells（HUVEC）.Methods：Human umbilical vein endothelial cells were cultured in vitro with adding TFA,and according to the concentration of total flavone of Abelmoschus manihot,HUVEC were divided into four groups：TFA 0 μg·ml-1 group（the control group）,TFA 5 μg·ml-1 group,TFA 10 μg·ml-1 group,TFA 20 μg·ml-1 group.After 72 hours,the rate of apoptosis and fas expression of HUVEC were determined by flow cytometry.Results：HUVEC apoptosis rates was 10.1% in control group,7.2% in TFA 5 μg·ml-1 group,3.9% in TFA 10 μg·ml-1 group,8.5% in TFA 20 μg·ml-1 group.Compared with the control group,difference of apoptosis rate was significant（P〈0.01）.The fas expression of HUVEC in different TFA conceutralion groups was decreased compared with control group（P=0.000,P=0.000,P=0.028）.Conclusions：Concentration of 5-20 μg·ml-1TFA can inhibit apoptosis of HUVEC,and the mechanism is related with reducing fas expression.
出处
《现代医学》
2012年第3期251-254,共4页
Modern Medical Journal
基金
江苏省中医药管理局课题(LZ09042)
关键词
人脐静脉血管内皮细胞
黄蜀葵花总黄酮
凋亡
FAS蛋白
human umbilical vein endothelial cells
total flavone of Abelmoschus manihot
apoptosis
fas expression
