摘要
用水蒸气蒸馏法从新疆一枝蒿中提取挥发油,利用GC-MS技术对其化学成分进行了分析和鉴定,并通过DPPH自由基清除体系和β-胡萝卜素漂白体系来评价新疆一枝蒿挥发油体外抗氧化作用。结果表明:共分离出49种组分,鉴定了其中的48种化合物,占总挥发油含量的89.82%,其主要成分为乙酸二氢香芹酯(31.54%)、2-羟基-3-(1-丙烯基)-1,4-萘醌(27.28%)等。抗氧化实验中,新疆一枝蒿挥发油在DPPH自由基清除体系中的IC50为0.195mg/mL,自由基清除能力相当于强抗氧化剂抗坏血酸(Vc)的70%～80%;在β-胡萝卜素漂白体系中的IC50为0.579mg/mL,抗脂质氧化能力相当于强抗氧化剂2,6-二叔丁基对甲酚(BHT)的85%～90%。表明新疆一枝蒿具有优异的抗氧化活性,且具有明显的量—效相关性。
The volatile oil was extracted from Artemisia rupestris L. by steam distillation. The components of the volatile oil were separated and identified by GC-MS. The antioxidant activity was also investigated with two different methods： the 1,1-Diphenyl-2-picrylhydrazyl （DPPH） radical scavenger and the 13-carotene-linoleic acid test. The results indicated that 49 kinds of components were separated and 48 components were identified among them.Accounting for about 89.82% of the total volatile oil. The main chemical constituents of the volatile oil from Artemisia rupestris L. are （-）-Dihydrocarvyl acetate（31.54%）, 2-hydroxy-3-（1-propenyl group）-1,4-napthoquinone（27.28%）, et al. Accounting for about 63.14% of the total volatile oil. The tested IC^0 of Artemisia rupestris L. volatile oil to DPPH was 0.195 mg/mL, the equivalent of a strong free radical scavenging capacity of antioxidants ascorbic acid （Vc） of 70% to 80%; In the 13-carotene-volatile oil linoleum acid system ICs0 of 0.579 mg/mL, lipid antioxidants is equivalent to strong antioxidant butylated hydroxy toluene （BHT） 85% to 90%. Therefore, Artemisia rupestris L. volatile oil had a good role in antioxidants bility. And had a concentration-effect relationship.
出处
《食品科技》
CAS
北大核心
2012年第7期213-217,共5页
Food Science and Technology
基金
新疆维吾尔自治区科技支疆项目(200991119)
新疆大学新疆生物资源基因工程重点实验室项目(XJDX0201-2009-04)
关键词
新疆一枝蒿
挥发油
化学成分
抗氧化活性
Artemisia rupestris L.
volatile oil
chemical composition
antioxidant activity
