摘要
为探讨绵羊肺腺瘤病毒囊膜蛋白(OPAV-Env)引起肺泡Ⅱ型上皮恶变的机理,将真核表达质粒pcDNA3.1-env用体内转染试剂经尾静脉转染到幼龄大鼠体内,用RT-PCR及免疫组化法检测绵羊肺腺瘤病毒囊膜蛋白在大鼠体内的表达,并采用SSCP法及基因克隆法结合序列分析检测H-ras基因是否发生突变。结果显示,绵羊肺腺瘤病毒囊膜蛋白在大鼠肺脏组织存在表达,但转染21d后和正常大鼠比较,未发现大鼠H-ras基因发生突变。
To explore the mechanism of alveolar type Ⅱ cell malignant transformation which was caused by the OPAV-env gene, the eukaryotic expression plasmid pcDNA3. 1-env was transfected into young rats with vivo transfection reagent through tail vein injection method. Then the RT-PCR and immunohisto- chemistry were used to detect the expression of pcDNA3.1-env plasmid in rats. Mutations of ras gene was detected by using methods of SSCP, gene clone and sequence analysis. The results showed that the plasmid pcDNA3.1-env was expressed in lung tissue of rats. 21 days after transfection, the ras gene mutation was not discovered compared with normal rats.
出处
《动物医学进展》
CSCD
北大核心
2012年第7期7-11,共5页
Progress In Veterinary Medicine
基金
国家自然科学基金项目(31060332)
关键词
绵羊肺腺瘤病毒
RAS基因
免疫组化
单链构象多态性
Ovine pulmonary adenomatosis virus
ras gene
immunohistochemistry
single strand conformation polymorphism
