摘要
目的 寻找新的高效杀伤活性的细胞 ,探讨脐血单个核细胞作为LAK细胞前体细胞的可行性和意义。方法 分离的脐血单个核细胞 ,分别用IL -2和IL -2 +IFNα诱导LAK细胞 ,并于培养后d 2 ,4,7,11,14分别测其细胞扩增数量和对K5 62细胞的杀伤活性。结果 于培养后d 11时 ,脐血单个核细胞的扩增倍数最高 ,两组分别为培养前的 2 0倍和 2 5倍 ,其对K5 62的杀伤活性分别为对照组培养前的 2 7倍和 3 5倍。结论 ①脐血单个核细胞是良好的LAK细胞的前体细胞 ;②其细胞数量和杀伤能力在培养的d 11达高峰 ;③IL -2 +IFNα组的细胞数量和杀伤活性优于单用IL
Objective To seek new activated killer cells, the feasibility of preparing LAK cell from umbilical blood was explored. Methods IL-2 and IL-2+IFN-α was used to induce LAK cells from umbilical blood mononuclear cells. The number and bio-activity of LAK cell were examined on the 2nd, 4th, 7th, 11th, 14th day after culture. Results The number of LAK cell and bio-activity of killing cancer cell (K562) reached their peaks on 11th day. The cell number and the cancer cell killing activity were greater in the IL-2+IFN-α group than those in IL-2 group.Conclusion ①The umbilical mononuclear cells is a good source for LAK cell;②The cell number and killing activity of umbilical blood LAK cells reach their peaks on the 11th day after culture;③The cell number and the killing activity are greater in IL-2+IFN-α group than those in the IL-2 group.
出处
《广东医学》
CAS
CSCD
2000年第5期381-382,共2页
Guangdong Medical Journal
