摘要
目的研究环维黄杨星D(CVB-D)对去甲肾上腺素(NE)诱导乳鼠心肌细胞损伤凋亡的影响。方法取SD大鼠乳鼠心肌细胞做原代培养,20μmol.L-1NE诱导心肌细胞凋亡模型,Giemsa染色TUNEL、DNA-Ladder凝胶电泳检测心肌细胞凋亡,免疫组化法检测caspase-3蛋白的表达,检测细胞LDH的外漏率,线粒体琥珀酸脱氢酶(MSDH)的活力,观察CVB-D的保护作用。结果模型组Giemsa染色形态学上出现典型的凋亡特征;DNA Ladder凝胶电泳出现典型的梯状条带;TUNEL染色法测定的凋亡率增高;Caspase-3的阳性表达率增高,细胞LDH的外漏率增加,MSDH活力降低。CVB-D明显的降低了细胞LDH的外漏率,提高了MSDH的活力,对心肌细胞的凋亡具有明显的保护作用。结论 CVB-D对心肌细胞凋亡和细胞的能量代谢障碍具有显著的保护作用。
Objective To investigate the cardio - protective effect of cyclovirobuxinum D（ CVB - D） on primary culture of cardiac myocyte treated with noradrenaline. Methods After adding CVB - D, the primary culture of cardiac myocyte was injured by 20μmol . L^-1 NE . The activity of lactic dehydrogenase （LDH） and mitochondrion succinic dehydrogenase （MSDH） were assayed. The percentage of cardiac myocyte apoptosis was observed by Giemsa staining, TUNEL,DNA - Ladder and immunohistochemistry was used for detection of Caspase - 3 protein. Results CVB - D significantly decreased the activity of LDH in culture supernatant, increased the activity of MSDH. Conclusion CVB - D can alleviate the disturbance of energy metabolism and decrease the per- centage of apoptosis of cardiac myocyte treated with noradrenaline. It has a significant protective effect on myocardial apoptosis.
出处
《时珍国医国药》
CAS
CSCD
北大核心
2012年第6期1358-1361,共4页
Lishizhen Medicine and Materia Medica Research
基金
国家自然科学基金(No.30701024)
贵州省高层次人才特助经费(TZJF-2006-13)
贵州省国际科技合作项目(黔科合外G字[2009]700115号)
贵州省科技攻关项目(黔科合SY字[2011]3010号)
