摘要
目的 :探讨胃癌组织中c erbB 2和 p5 3基因的表达及其意义。 方法 :应用荧光原位杂交技术对 5 5例胃癌及移行区、正常区组织的常规石蜡包埋标本进行检测。结果 :c erbB 2和p5 3基因表达的阳性率分别为36 36 %和 45 45 % ,移行区为 10 91%和 3 6 4% ,正常粘膜区为 3 6 4%和 0 ,三区间差异均有极显著意义 (P值均 <0 0 1)。在肠型和弥漫型胃癌中 ,c erbB 2阳性率分别为 5 1 6 1%和 16 6 7% ,p5 3阳性率分别为 2 5 81%和70 83% ,其在两型间的差异有显著性意义 (P <0 0 5 )。c erbB 2和 p5 3基因表达与胃癌的组织学分级有关 (P <0 0 5或P <0 0 1) ;c erbB 2基因表达阳性与胃癌的淋巴结内转移有关 (P <0 0 5 )。结论 :c erbB 2基因扩增和p5 3基因突变与胃癌的发生、类型、分化和转移有关 ,其测定可有助于确定肿瘤的生物学行为。
Objective:To investigate the expression of c erbB 2 and p53 gene in gastric carcinoma Method:The C erbB 2 and p53 expressions were dedected by in situ hybridization (FISH) for 55 cases of gastric carcinoma tissues, their transitional region tissues and normal tissues of formalin fixed, paraffinembedded sections Results:The positive rate of c erbB 2 and p53 were 36 1%and 45 5%in cancer tissues, 10 91%and 3 64%in transitional regions, 3 64%and 0 in normal regions respectively There were highly significant differences among the three regions (all P< 0 001) In intestinal type and diffuse type gastric carcinoma, the incidences of c erbB 2 were 51 61%and 16 37%, and of p53 were 25 8%and 70 83%respectively,which was significantly different between two types (all P< 0 05) The expression of c erbB 2 and p53 gene were also correlated with the histological grades of the tumors (P< 0 01 or 0 05) The positive of C erbB 2 gene was related to lymph node metastasis of gastric carcinoma (P< 0 05) Conclusions:The amplification of c erbB 2 gene and mutation of p53 are related to genesis type, differentiation and metastasis, and thus the detection of these gene expression might be useful in evaluating the biological behavior of the tumors
出处
《癌症》
SCIE
CAS
CSCD
北大核心
2000年第5期426-428,共3页
Chinese Journal of Cancer
基金
广州市科委基金!项目 (No:1999-Z -10 2 - 10 )资助
