摘要
目的研究FISH技术检测慢性淋巴细胞性白血病(CLL)染色体异常的价值。方法用3、12、18号标记有不同荧光素的染色体着丝粒探针,用荧光原位杂交技术(FISH)检测25例CLL,并和常规细胞遗传学(Conventionalcytogenetics,CC)检测方法即R显带法进行比较,以明确何种方法对CLL染色体异常检出更敏感可靠。结果25例CLL患者中,常规细胞遗传学检出+3,+12,+185例,检出率为20%;其中:+123例,+3、+121例,+3、+12、+181例;FISH方法检出8例异常(32%),+34例;+126例;+181例。CC可检出不确定异常:t(5;15)1例;3q-,18p+1例;4q+13q-1例;+19,-221例。结论FISH方法是检测CLL患者染色体异常的有效技术,可提高染色体异常检出率,明显优于常规显带法。
Aim To investigate the value of detection rate of chromosome aberration in CLL by FISH. Methods Centromere probes for chromosome 3,12 and 18 were used to detect trisomy 3.12 and 18 in 25 cases with CLL. Meanwhile its results were compared with that of conventional cytogenetic R banding. Results From 25 cases of CLL,CC can detect 5 cases with abnormalities（20% ） ,including ＋ 12 3 cases, ＋ 3, ＋ 12 lcase, ＋ 3, ＋ 12, ＋ 18 1 case,while 8 cases（32% ）can be detected by FISH, ＋ 3 4 cases, ＋ 12 6 eases, ＋ 18 1 case. CC method can detect uncertain abnormalities as t （5 ; 15 ） 1 case, 3 q -, 18p ＋ 1 case ;4q ＋ , 13 q - I case; ＋ 19, - 22 1 case. Conclusion Compared with CC, FISH can elevate the detection rate of chromosome aberration and obviously superior to CC,is the valid method to detect chromosome aberration of CLL.
出处
《安徽医药》
CAS
2007年第1期54-55,共2页
Anhui Medical and Pharmaceutical Journal
