摘要
目的:探讨慢性淋巴细胞白血病(CLL)患者外周血CD4+CD25+调节性T细胞(Treg细胞)表达及其与预后的相关性。方法:采用流式细胞仪检测50例健康者(对照组)及30例CLL患者(CLL组)治疗前后CD4+CD25+Treg、CD4+CD25+Foxp3+Treg水平。结果:初诊CLL组患者外周血T淋巴细胞数量、CD4+CD25+Treg、CD4+CD25+Foxp3+Treg水平均显著高于对照组,差异有统计学意义(P<0.05)。治疗后,CLL组患者外周血T淋巴细胞数量、CD4+CD25+Foxp3+Treg水平显著降低,与初诊时比较差异有统计学意义(P<0.05),但仍高于对照组(P<0.05)。CLL患者中,Binet临床分期A期患者CD4+CD25+Foxp3+Treg水平显著低于B/C期患者,差异有统计学意义(P<0.05)。Spearman相关分析结果显示,Binet临床分期与CD4+CD25+Foxp3+Treg水平呈正相关(r=0.511,P<0.05)。结论:CD4+CD25+Foxp3+Treg水平可能是评估CLL患者预后的有效指标。
Objectlves：To evaluate the sifnificance of CD4＋ CD25＋ regulatory T cells for the prognosis of chron- ic lymphocytic leukemia. Method：CD4＋ CD25＋ Treg and CD4＋ CD25＋ Foxp3＋ Treg level before and after treatment of 50 cases healthy subjects and 30 cases CLL patients were detected by flow cytometry. Resultt The peripheral blood T lymphocytes, CD4＋ CD25＋ Treg and CD4＋ CD25＋ Foxp3＋ Treg levels of initial diagnostic patients were significantly higher than that of healthy group（P〈0.05）. After treatment,the number of peripheral blood T lym phocytes and CD4＋ CD25＋ Foxp3＋ Treg levels of CLL patients were significantly reduced, compared with initial diagnosis（P〈0.05）, but still higher than that of healthy group （P〈0.05）. In CLL group, the CD4＋ CD25＋ Foxp3＋ Treg of patients with Binet stage A significantly lower than patients with Binet stage B/C（P〈0.05）. Spearman correlation analysis showed that Binet stage was positively correlated with CD4＋ CD25＋ Foxp3＋ Treg levels （r= 0. 511, P〈0.05）. Conclusion.. CD4＋ CD25 ＋ Foxp3＋ Treg may be an effective index to evaluate the prognosis of CLL patients.
出处
《临床血液学杂志》
CAS
2015年第2期221-223,共3页
Journal of Clinical Hematology
