摘要
目的建立测定祖师麻叶中祖师麻甲素含量的高效液相色谱法。方法采用Ultrasphere C18柱(250 mm×4.6 mm,5μm),流动相为甲醇-0.4%磷酸(20∶80),流速1.0 mL/min,检测波长为327 nm。结果祖师麻甲素进样量在0.102～0.510μg范围内与峰面积具有良好的线性关系,r=0.999 9;平均加样回收率为99.03%,RSD为1.16%(n=6)。结论所用方法简便、准确,灵敏度高,重现性好,可作为控制祖师麻叶的质量控制方法。
Objective To establish a RP-HPLC method for the quantitative determination of daphnetin in Daphnes folium.Methods The separation was performed on the Ultrasphere C18 column（250 mm×4.6 mm,5 μm）with the mobile phase of methanol-0.4%phosphoric acid（20 ∶80）at a flow rate of 1.0 mL/min.The UV detection wavelength was 327 nm.Results The linear range of daphnetin was 0.102-0.510 μg,r=0.999 9.The average recovery rate was 99.03%with RSD of 1.16 %（n=6）.Conclusion This method is simple,accurate and sensitive with good reproducibility,which can be used as the quality control method of Daphnes folium
出处
《中国药业》
CAS
2012年第12期22-23,共2页
China Pharmaceuticals
基金
全军中医药专项课题资助项目
项目编号:10ZYX209
