摘要
目的研究荧光定量PCR技术检测宫颈感染HPV的方法。方法选取已确诊患有宫颈疾病的50例妇女作为试验组;再选取同时间段内的50例健康妇女作为对照组,采用荧光定量PCR检测技术对两组妇女进行HPV16、HPV18检测,分析两组妇女的检测结果,研究荧光定量PCR技术检测宫颈感染HPV的临床意义。结果试验组的50例妇女中,HPV16检测结果呈阳性的有6例,所占比例为12%,HPV18检测结果呈阳性的有2例,所占比例为4%。对照组的50例妇女中,HPV18检测结果呈阳性的有1例,所占比例为2%,未出现HPV16检测结果呈阳性的病例。结论荧光定量PCR技术检测宫颈感染HPV的方法简洁准确,医务人员应该积极利用并不断探索更好的诊治方法。
Objective To study the fluorescent quantitative PCR technology to detect cervical infection with HPV method. Methods The selection has been diagnosed with cervical disease 50 cases of women as the experimental group, then select the time period of the healthy women50 cases as control group, using fluorescence quantitative PCR detection technology on the two groups of women were HPV16, HPV18 test, analysis of test results of two groups of women, research of fluorescence quantitative PCR technology to detect cervical HPV infection method of. Results The experimental group 50 cases of women, HPV16 monitoring results were positive in 6 patients, the proportion of 12%, HPV18 test results were positivein 2 cases, the proportion of 4%. A control group of 50 cases of women, the results of HPV^s positive in 1 cases, the proportion of 2%, no HPV16 test results positive phenomenon. Conclusion The fluorescent quantitative PCR assay for detection of cervical HPV infection method is simple and accurate, the medical staff should actively use and continue to explore better methods of diagnosis and treatment.
出处
《当代医学》
2012年第19期86-87,共2页
Contemporary Medicine
