摘要
目的建立一方便、实用和易于操作的轻度水肿性胰腺炎模型以更好地对急性胰腺炎的发病机制及防治措施进行研究。方法采用Wistar大鼠 ,以Caerulein作为急性水肿性胰腺炎的刺激诱导剂 ,将Caerulein预灌入一特制的渗透压泵 (Alzet)中 ,再将该泵植于大鼠左下腹 ,以静脉输入方式 ,将Caerulein由股静脉缓慢输入血液而诱发胰腺炎。结果由病理组织检查证实 ,以Caerulein(312.5mg/L,8μl/h)静脉灌注6h即显示明显组织水肿 ,大体检查胰腺各小叶间被清亮的胶冻样液体分隔 ,光镜检查显示胰腺间质水肿和明显的空泡化 ,但无炎症浸润 ;而仅以生理盐水灌注者则未见胰腺组织学的明显变化。结论采用渗透压泵植入大鼠腹部皮下以Caerulein缓慢静注能够可靠地诱导轻度水肿性胰腺炎 (组织学证实 ) ,该技术具有操作方便 ,效果可靠 ,并减少对动物干扰性操作的优点。
Objective To establish a refined model of intravenous Caerulein-induced edematous pancreatitis in order to study the pathogenesis and therapeutic protocols of the disesase. Methods Wistar rat was employed. The osmotic pump pre-filled with Caerulein and saline was implanted into a subcutaneous pocket in the left iliac fossa of the animal. Caerulein was then infused via femoral vein to induce pancreatitis. Results Pathological examination revealed obvious tissue edema of the pancreas in the rat 6 hours after Caerulein infusion. The gross appearance of the pancreas exhibited edema with a clear ‘gel-like’ fluid separating the pancreatic lobules. Interstitial edema and marked vacuolization without inflammatory infiltrations in the Caerulein-infused rats were observed with light microscopy. There was no remarkable pathological changes in the pancreas of rats infused with normal saline. Conclusion The advantages of using implantable osmotic pump to infuse Caerulein to rats are the facilitation of animal handling, unrestriction of animal movement in cages and reduction in time lost due to acclimatization of the rat to new housing conditions.
出处
《第三军医大学学报》
CSCD
北大核心
2000年第4期345-348,共4页
Journal of Third Military Medical University
基金
WHO1998/1999年度基金
