摘要
目的研究不同温度下白纹伊蚊对登革病毒的易感性差异。方法登革2型病毒人工经口感染白纹伊蚊，冻麻挑出吸饱血的雌蚊放入新的蚊笼，分别置于18％、21℃、26℃、31℃、33℃和36℃的人工气候箱中饲养，经外潜伏期后，解剖蚊虫，间接免疫荧光法分别检测蚊虫的头部、唾液腺和胸腹部内的登革2型病毒抗原，并计算蚊虫的播散感染率。结果18℃白纹伊蚊感染率最低，胸腹部感染率为8％，头部和唾液腺未检出。31℃胸腹部感染率最高为82％，头部和唾液腺感染率33℃时最高。36℃各部位感染率均较33℃下降。18℃的播散感染率最低为0，36℃播散感染率最高，达到了100％。结论白纹伊蚊感染率随温度的升高，呈现先升后降趋势。18％可能是白纹伊蚊传播登革病毒的最低温度。播散感染率随温度的升高逐渐升高。
Objective To investigate the effect of different temperature on the susceptibility of Aedes albopictus for transmission of dengue 2 virus. Methods Mosquitoes were orally infected with dengue virus type 2 suspension, then fully blood-engorged mosquitoes were separated and transferred to new cartons and sustained at 18,21, 26,31,33℃ and 36℃ with 10% sucrose. Immunofluorescence assays were used to detect antigen of dengue virus type 2 in the head, salivary gland and thorax-abdomen, and calculated the disseminated infection rate of mosquitoes. Results For mosquitoes held at 18℃ ,the infection rate of thorax- abdomen was 8% ,infection in head and salivary gland were not detected until 25d. Maximum infection rate of thorax-abdomen was attained at 31℃ ,the highest infection rate of head and salivary gland existed at 33℃ , however, the infection rate of mosquitoes at 36℃ was significantly decreased compared with that at 33℃. The lowest disseminated infection rate was zero at 18% , while the highest disseminated infection rate was 100% at 36℃. Conclusion As the temperature went up,the infection rate showed a tendency of ascending first and descending later. Dengue virus might not be transmitted by Aedes albopictus when temperature was below 18℃. The disseminated infection rate increased gradually with the increase of temperature.
出处
《中华实验和临床病毒学杂志》
CAS
CSCD
2012年第3期186-188,共3页
Chinese Journal of Experimental and Clinical Virology
基金
基金项目：国家“十一五”科技支撑计划项目（No.2007BAC03A02）
国家科技重大专项项目（2008ZX10004-011）
福建省社会发展重点项目（2010Y0010）
福建省卫生厅青年科研项目（2011-2-26）
