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初始生物素含量波动时谷氨酸发酵关键酶系的酶活变化模式 被引量:3

Activities changes of key enzymes in glutamate fermentation in response of various initial biotin contents
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摘要 谷氨酸发酵培养基中的生物素初始含量会随玉米浆来源、批次的不同出现波动,进而影响谷氨酸发酵性能和稳定性。本文研究分析了谷氨酸发酵中,初始生物素含量正常/不当,特别是初始含量不当、采取补救措施条件下的丙酮酸、异柠檬酸和α-酮戊二酸代谢节点处关键酶的活性变化。生物素匮乏时,催化主代谢途径的关键酶活性均被弱化,特别是α-酮戊二酸脱氢酶完全失活,能量代谢主要靠乙醛酸循环维持。当发现生物素匮乏并补加生物素后,中心代谢途径关键酶——丙酮酸脱氢酶的活性恢复到正常水平,TCA重新成为主要供能途径。生物素过量时,丙酮酸羧化酶和谷氨酸脱氢酶活性基本不变,而其他关键酶均被激活。当发现生物素过量并添加吐温40后,丙酮酸脱氢酶和异柠檬酸脱氢酶依旧保持很高活性,而α-酮戊二酸脱氢酶和异柠檬酸裂解酶的活性则下降到正常水平。采用上述补救措施可以挽救因初始生物素匮乏或过量所引起的错误发酵,终酸浓度均可恢复到对照(生物素亚适量)水平(75~80g·L-1)。另外,对生物素初始含量和吐温添加进行复合式调控,终酸浓度可进一步提高,达到87g·L-1,比对照提高8.8%。 In glutamate fermentation,initial biotin content in the medium varies with the corn slurry originating sources or even production batches,and this variation affects fermentation performance and stability.In this study,activities changes of the key enzymes at metabolic nodes of pyruvate,isocitrate and α-ketoglutarate when initial biotin content was at normal/improper levels,particularly when initial biotin content was at improper levels but adopting faults-rescue measures during fermentation were carefully investigated.When initial biotin was in shortage,all activities of the key enzymes responsible for catalyzing the main glutamate synthesis route were weakened,in particular,the activity of α-oxoglutamate dehydrogenase complex was inactivated and energy metabolism completely relied on glyoxylate cycle.When biotin in shortage was determined and biotin was supplemented,the enzyme responsible for the central metabolic route,pyruvate dehydrogenase returned to the normal level,and TCA cycle turned to be the main energy metabolism route once again.On the other hand,when initial biotin was in excess,all activities of the key enzymes were activated except pyruvate carboxylase and glutamate dehydrogenase.When biotin in excess was determined and Tween 40 was supplemented,the activities of two key enzymes responsible for glutamate synthesis,pyruvate dehydrogenase and isocitrate dehydrogenase,remained at high levels.At the same time,activities of α-oxoglutamate dehydrogenase complex and isocitrate lysase declined to the normal levels.The final glutamate concentration of the failure-likelihood fermentations could be recovered back to the control level(75—80 g·L-1)by using the relevant rescue measures.Furthermore,final glutamate concentration could be further increased and reach 87 g·L-1,which was over 108.8% of that obtained in the control case,when adopting the "two-stage" optimal operation mode based on combined adjustment of initial biotin content and Tween 40 addition.
出处 《化工学报》 EI CAS CSCD 北大核心 2012年第7期2188-2194,共7页 CIESC Journal
基金 国家高技术研究发展计划项目(2006AA020301-11) 国家重点基础研究发展计划项目(2007CB714303)~~
关键词 代谢分析 谷氨酸发酵 酶学分析 生物素 吐温 metabolic analysis glutamate fermentation enzymatic analysis biotin Tween
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