摘要
考察了大孔树脂对紫苏茎提取液中总黄酮的吸附性能,优化了吸附工艺参数。首先对D-101、AB-8、DM130、ADS-7和ADS-17共5种大孔树脂的静态吸附量和解析率进行了实验,选择AB-8为最佳吸附树脂;静态吸附表明,3h内吸附即可达到平衡。还考察了上样速率、上样质量浓度、洗脱液乙醇质量分数和洗脱速率对分离的影响,结果表明优化的条件为:上样速率为1BV/h,上样质量浓度为0.15mg/mL,洗脱液乙醇质量分数为70%,洗脱流速为2BV/h。在此条件下,总黄酮洗脱率为93.56%,总黄酮纯度可提高4.5倍。
The absorption property of pefilla frutescens total flavonoids on macroporous resins were studied, and the adsonrption parameters were optimized. Among five types of macroporous adsorption resin chosen, the results showed that AB-8 type maeroporous resin is suitable for separating Pefilla flavonoids. Static adsorption showed that adsorption equilibrium can be reached in 3 h. Then the effects of sampling concentration, sampling rate, eluting eoneentrantion and velocity on the separation were examined, the optimun conditions was： sampling rate 1 BV/h, sampling concentration 0. 15 mg/mL, eluting concentration 70~, eluting velocity 2 BV/h. Under this condition, the elution rate of flavone is 93.56%, the purity of flavone can be improved 4.5 times.
出处
《浙江科技学院学报》
CAS
2012年第3期232-236,共5页
Journal of Zhejiang University of Science and Technology
基金
浙江省科技计划项目(2009C32060)
关键词
紫苏茎
总黄酮
大孔树脂
吸附
stems of Pefilla frutescens
total flavonoids
macroporous resin
adsorption
