摘要
为克服酸碱法水解猪皮胶原对氨基酸的破坏,拟利用胰蛋白酶水解猪皮胶原来获得更多的氨基酸.研究发现,随着胰蛋白酶含量增加,水解度增加,当pH 7～8时,胰蛋白酶对猪皮水解的水解度达到最大值;水解反应的开始反应速度较大,30分钟后反应速度降低.胰蛋白酶水解猪皮的最佳条件为:pH 7.5,水解温度48℃,酶与猪皮比40mg/g,底物浓度25%,水解时间2h.在此条件下,猪皮的水解度可达16%.
In this work, trypsin was used to hydrolyze pigskin in order to avoid the destruc- tion of collagen polypeptide during acid-alkali hydrolysis process. It was found that the hy- drolysis degree increased with the increase of the trypsin content. The maximum hydrolysis degree of pigskin was realized at pH 7~8. The initial rate of the hydrolysis reaction was large while it decreased after reacting for 30 minutes. The optimum condition of the hydroly- sis of pigskin by trypsin was as follows, pH 7. 5, hydrolysis temperature 48 ℃, ratio of trypsin 40 mg/g, substrates concentration 8%, hydrolysis time 2 h. Under this condition, the hydrolysis degree of pigskin could reach 16%.
出处
《陕西科技大学学报（自然科学版）》
2012年第3期23-25,29,共4页
Journal of Shaanxi University of Science & Technology
基金
陕西省科学院科技计划项目(2009K-02)
陕西省科学院科技计划项目(2006K-19)
关键词
胰蛋白酶
猪皮
胶原多肽
水解
trypsin
pigskin
collagen polypeptide
hydrolysis
