摘要
目的:探讨自体松质骨和骨髓间充质干细胞联合移植治疗股骨头缺血性坏死的可能机制,为该方法应用于临床提供依据。方法:兔龄4～5个月的新西兰大耳白兔24只,雌雄不限,随机分为2组,每组12只。采用液氮冷冻股骨头负重区的方法造成股骨头缺血坏死模型后,A组进行自体松质骨移植,B组进行自体松质骨和骨髓间充质干细胞联合移植。每组术后2、4、6、8周分别处死3只动物,制作股骨头标本,进行X线检查和组织学观察,并对术后8周的股骨头标本进行扫描电镜观察。结果:①X线观察结果。A组术后2周时,有2个标本钻孔区密度不均匀,钻孔边缘清晰可见;4周时,有3个标本钻孔区密度增高不显著,钻孔边缘出现硬化线;6周时有4个标本钻孔区显示有骨吸收现象,出现股骨头关节面塌陷;8周时有3个标本骨吸收现象明显,而骨小梁结构不清晰。B组术后2周,股骨头形态均正常,钻孔区有密度增高影,质地均匀,钻孔边缘尚可分辨;4周时股骨头形态均正常,钻孔区密度进一步升高,质地均匀,钻孔边缘变得模糊;6周时股骨头形态仍正常,钻孔区密度高而均匀,钻孔边缘出现放射状骨小梁接合现象;8周时钻孔区密度接近周围骨质,股骨头内出现张力性和压力性骨小梁,钻孔边缘交界区骨小梁接合良好。②组织学观察结果。A组术后2周时,移植骨发生坏死,4个标本出现再血管化,移植区出现破骨和成骨细胞,成骨细胞的数目较少,移植骨周围附加新生骨较薄,有少量连接现象;另2个标本局部新生血管较少,成骨细胞募集不良,新生骨组织的形成较少,无连接现象。术后4周时,3个标本新生骨增加,连接现象增多,出现骨塑形;另3个标本移植骨无明显修复现象。术后6周时,有2个标本新生骨量增加,有骨塑形现象;另4个标本移植骨无明显修复表现,出现骨吸收现象。术后8周时,3个标本可见到塑形的板层骨小梁和�
Objective：To investigate the mechanism of action of spongy bone autografting combined with bone marrow mesenchymal stem cells（BMSCs） transplantation on the experimental avascular necrosis of femoral head（ANFH） for the purpose of application in clinic. Methods：Twenty-four New Zealand white rabbits ,4 to 5 months old, were divided into 2 groups randomly, 12 cases in each group. After the rabbit model of ANFH were built by freezing the weight loading region of femoral head with liquid nitrogen, rabbits in group A were adminis- trated with spongy bone autografting, while rabbits in group B were administrated with spongy bone autografting combined with BMSCs transplantation. Three rabbits selected from each group were sacrificed 2,4,6,8 weeks after the transplantation for making specimens of femoral head, which were observed with radioscopy, histological examination. Moreover, those specimens obtained 8 weeks after the transplantation were observed with scanning electron microscope. Results： Radiological observations：In group A, two specimens presented uneven density in drilled area and clear images of drilled holes 2 weeks after operation, sclerosis line was showed at the margin of drilled area in 3 specimens and increase in density were not significant in drilled area 4 weeks after operation, bone resorption of drilled area and femoral head collapse appeared in 4 specimens 6 weeks after operation, three specimens presented obvious bone resorption and disordered structure of trabeculae of bone 8 weeks after operation. In group B, the shape of femoral head was normal and the bone density increased uniformly in the drilled area while the margin of drilled area could be distinguished 2 weeks after operation ,the shape of femoral head was normal and the bone density further increased uniformly in the drilled area and the margin of drilled area became obscured 4 weeks after operation, the shape of femoral head was still normal and the bone density was high and uniform in the drilled area and radial conjunc
出处
《中医正骨》
2012年第6期13-17,共5页
The Journal of Traditional Chinese Orthopedics and Traumatology
基金
河北省科技厅科技攻关项目(2007276171)
关键词
股骨头坏死
间质干细胞移植
骨移植
动物实验
兔
Femur head necrosis
Mesenchymal stem cell transplantation
Bone transplantation
Animal experimentation
Rabbits
