摘要
目的:探讨牛蒡子苷元对结肠癌细胞系SW480增殖的影响及相关机制。方法:以结肠癌细胞系SW480为研究对象,通过MTT比色法检测牛蒡子苷元对SW480细胞生长速度和倍增时间的影响;通过软琼脂克隆形成实验观察牛蒡子苷元对SW480细胞软琼脂集落形成率的影响;应用RT-PCR技术探讨牛蒡子苷元对SW480细胞增殖的相关机制。结果:①不同浓度牛蒡子苷元处理的SW480细胞生长速度明显减慢,倍增时间显著延长,并且呈现一定的浓度和时间依赖性;②SW480亲本细胞和牛蒡子苷元(20 mg/L)处理24 h的SW480细胞的克隆形成率分别为24.933%和4.533%(P<0.01),与SW480亲本细胞相比,牛蒡子苷元(20 mg/L)处理24小时的SW480细胞形成的克隆体积较小;③与SW480亲本细胞相比,20 mg/L牛蒡子苷元处理的SW480细胞中,cyclin A基因表达没有明显变化,p21基因表达上调,而cyclin B、cyclin E基因表达下调。结论:①牛蒡子苷元可抑制SW480细胞的增殖能力。②诱导p21基因表达上调,cyclin B和cyclin E基因表达下调是牛蒡子苷元抑制SW480细胞增殖的可能机制。
Objective： To investigate cell effect of arctigenin on cell proliferation of colorectal cancer cell line SW480 and its mechanism. Method： Effects of arctigenin on growth rate and doubling time of cell line SW480 were detected with MTT method; influence of arctigenin on clone formation rate of cell line SW480 was observed through clone formation assay in soft agar; mechanism of arctigenin on the proliferation of cell line SW480 was studied with RT-PCR assay. Result： （1） The results showed that growth speed of cell SW480 obviously lowered and doubling time remarkably prolonged in the presence of different concentrations of arctigenin, both appeared a dose-and-time-depen- dent manner; Q Clone-forming efficiency of cells induced with 20mg/L arctigenin for 24h was 4.533%, lower than 24.933% of blank control group （P〈0.01）. The cloning volume of cells induced with 20mg/L arctigenin for 24h was smaller; （3） RT-PCR assay showed that expression of p21 gene up-regulated, expression of cyclin B and E genes down-regulated, but cyclin A was with no changes. Conclusion： （1） This study provides a preliminary proof of arctigenin＇s inhibition on proliferation of SW 480 ceils. （2） Up-regulation of p21 and down-regulation of cycUn B and E might explain the proliferation inhibition.
出处
《西部中医药》
2012年第6期17-20,共4页
Western Journal of Traditional Chinese Medicine
关键词
结直肠癌
牛蒡子苷元
增殖
colorectal cancer
arctigenin
proliferation
