摘要
目的:观察三七总皂苷对腹膜透析失超滤大鼠模型腹膜形态和超滤功能的影响。方法:实验大鼠随机分为12组:其中高浓度葡萄糖3组(1.5%、2.5%、4.25%);脂多糖(LPS)3组(0.1mg/L、1mg/L、10mg/L);金黄色葡萄糖球菌灭活菌液2组(1011/mL、1015/mL);三七总皂苷干预3组(2.5%葡萄糖溶液+三七总皂苷溶液、1mg/L脂多糖+三七总皂苷溶液、1011/mL金黄色葡萄球菌灭活菌液+三七总皂苷溶液)和正常组。各组大鼠每天腹腔注射相应溶液20mL,20天后做腹膜平衡试验并行腹膜组织病理检查。结果:三七总皂苷可改善高浓度葡萄糖、脂多糖和金黄色葡萄球菌灭菌液引起的腹膜失超滤;高浓度葡萄糖、脂多糖和金黄色葡萄球菌灭菌液均可增加腹膜毛细血管密度,造成毛细血管充血,毛细血管壁增厚,腹膜中炎性细胞浸润。三七总皂苷可以降低腹膜毛细血管密度,明显扩张血管,腹膜毛细血管壁明显增厚、炎性细胞浸润减少。结论:三七总皂苷可改善腹膜超滤功能,其引起的主要腹膜病理改变为腹膜毛细血管密度下降、血管明显扩张,血管壁明显增厚、炎性细胞浸润减少。
Objective ：To observe the influences of Panax pseudo - ginseng on peritoneal morphous and uhrafihration function in uhrafihration failure rat models during peritoneal dialysis. Methods： Randomly put the rats into 12 groups, in- cluding normal group,three high concentrations of glucose groups （1.5% ,2.5% ,4.25% ）, three lipopolysaccharide （ LPS ） groups （ 0. 1 rag/L, 1 mg/L, 10mg/L ）, two inactivated Staphylococcus aureus groups （ 1011/mL, 1015/mL ） and three Panax pseudo- ginseng groups including Panax pseudo -ginseng associated with 2.5% glucose, Panax pseudo - ginseng associated with 1 mg/L LPS and Panax pseudo -ginseng associated with 1011/mL inactivated Staphylococcus an- reus. 20mL fluid was injected into the rats＇ abdominal cavities each day for 20 days. Then make PET and observe the pathological changes in peritoneum. Results ： Panax pseudo - ginseng could improve uhrafiltration failure led by high con- centration of glucose, LPS and Staphylococcus aureus, high concentration of glucose, LPS and inactivated Staphylococcus aureus could increase peritoneal capillary density, make capillary hyperemia and the walls of capillary thickening and in- flammatory cell infiltrate into peritoneal interstitium. Panax pseudo - ginseng could decrease peritoneal capillary density, obviously distend vessels,obviously thicken the walls of capillaries and decrease inflammatory cell infiltrate. Conclusions： Panax pseudo - ginseng could improve ultrafihration function of peritoneum and Panax pseudo - ginseng could decrease capillary density, distend vessels, thicken the walls of capillaries and decrease inflammatory cell infiltrate.
出处
《中华中医药学刊》
CAS
2012年第6期1246-1248,I0010,共4页
Chinese Archives of Traditional Chinese Medicine
基金
国家自然科学基金资助项目(30801498)
关键词
三七总皂苷
腹膜透析
超滤
腹膜
Panax pseudo - ginseng
peritoneal dialysis
ultrafiltration
peritoneum
