摘要
目的探讨胰岛素对2型糖尿病大鼠种植体周围骨形成蛋白-2表达的影响及意义。方法将SD大鼠30只,随机分为3组正常组、糖尿病组、糖尿病+胰岛素组。糖尿病模型采用高糖高脂饮食加小剂量链脲佐菌素诱导建立;种植模型于大鼠胫骨近骺端植入纯钛种植体(直径1.3mm,长度5mm)。糖尿病+胰岛素组每日定时给予胰岛素皮下注射,正常组、糖尿病组注射等量生理盐水。12周后测量血糖水平及体重,拍摄x线片,取胫骨标本,免疫组化法测定种植体周围骨组织骨形成蛋白-2(BMP-2)的表达水平。结果糖尿病+胰岛素组种植体周围BMP-2表达水平高于糖尿病组,差异有统计学意义(P<0.01)。结论胰岛素可使糖尿病大鼠种植体周围BMP-2表达水平提高,可能促进种植体周围的骨形成。
[ Objective] To investigate the expression and meaning of the morphogenetic protein - 2 that influenced of insulin in type 2 diabetic rat peri - implant bone. [ Methods ] 20 type 2 diabetic rats were divided into 3 groups normal group, diabetic group and diabetic ＋ insulin group. Type 2 diabetic model rats were fed on high - glucose - high - fat diet and injected by low dose of streptozotocin （STZ）. Implant model rats were implanted the titanium implant （ 1.3mm ×5mm） o Diabetic ＋ insulin group inject with insulin everyday. And the diabetic group and normal group inject with equally physiological saline. 12weeks later, blood glucose levels and weight of the rats were collected, X rayed. Then the rats were killed tibia and serum were collected. Immunohistochemical technique was used to assess the expression of BMP -2 in bone tissue around the implant. [ Results] BMP -2 in treatment rats peri -implant bone is more than diabetic group. The difference was statistically significant（ P 〈0.01 ）. [ Conclusion] Insulin can improve morphogenetic BMP - 2 in type 2 diabetic rat peri - implant bone. , maybe can improve the bone formation around the implant.
出处
《中国医学工程》
2012年第4期40-41,共2页
China Medical Engineering
