摘要
霍乱是由O1血清群和O139血清群霍乱弧菌引起的急性肠道传染病,其特征为发病急、传播快、波及范围广,是能引起大流行的烈性传染病(即属甲类传染病)之一,以夏秋季节发病多见,传染源多为霍乱病人和病原携带者,通过其粪便污染水源、食物和周围环境等,经口传入引发疾病。人类是霍乱弧菌的易感者,因此快速准确的实验室诊断是及时快速发现疫情,控制疫情发生、发展的重要关键[1-3]。近年来,国内许多专家及实验研究人员,就霍乱弧菌的快速检测围绕着细胞学和分子生物学进行多方面的深入研究。其中采用以细胞学为基础的高速离心法、免疫学方法、免疫荧光菌球法、胶体金免疫层析法、快速测试纸条法和以分子生物学为基础的脉冲场电泳法、PCR法等多种方法来提高检出的灵敏度和特异性,从而达到快速检测的目的。下面就近几年来国内报道的几种对霍乱弧菌的快速检测法做如下综述。
Cholera is serum group and O139 by O1 serum group of choleraic vibrio causing the acute intestinal infection,and is characterized by come on urgent, transmission is fast, spread to a wide range, can cause big popular deadly infectious diseases, one of a summer to see more throttling behavior, source for cholera patients more and pathogen carriers, through its waste water, food and the surrounding environment, etc. , the mouth to cause disease. We humans are the choleria vibrio susceptible persons, so fast and accurate laboratory diagnosis is to quickly find that the outbreak, control the outbreak,development of the important key^[1-3]. In recent years, many domestic experts and experimental research personnel, choleraic vibrio fast detection around cytology and molecular biology carry on various research. Use among them to the high speed centrifugation based cytology, immunological methods, immunofluorescence bacteria ball method,colloidal gold immune chromatography,rapid test note method and molecular biology in the basis of pulse field e- lcctrophoresis method,PCR method and so on many kinds of methods to improve the detection sensitivity and specificity,so as to a- chieve the purpose of fast detection. Here is the domestic reported in recent years some kinds of rapid choleria vibrio do the following method were reviewed.
出处
《哈尔滨医药》
2012年第3期231-231,233,共2页
Harbin Medical Journal
关键词
霍乱弧菌
快速检测
概况
Cholerae vibrio
Rapid detection
General situation
