摘要
霍乱弧菌快速、准确的检定是控制霍乱流行的重要手段。本研究建立了ctxA-PCR快速检测鉴定方法,并用于新疆霍乱弧菌O139腹泻暴发的病人诊断和环境水体的污染状况评价,取得了较理想的效果。该方法灵敏度高、特异性好、被扩增样品中含4个以上活菌细胞即可检出,为霍乱防治和分子流行病学研究提供了又一有效手段。
Rapid detection and
identification of the pathogen are the key measures for cholera control and prevention. In this
study,a method of ctxA-PCR was established and was used to diagnose the patients in the
outbreak of O139 cholera occured in Xinjing, China,and also be used to evaluate the
environmental water in the epidemic area. The results showed that this method was efficient
to identify the strains possessing ctxA gene,and was very sensitive and specific. This method
would be very useful for the disease control and molecular epidemiological study of cholera.
出处
《中华微生物学和免疫学杂志》
CAS
CSCD
北大核心
1994年第6期376-379,共4页
Chinese Journal of Microbiology and Immunology
基金
国家自然科学基金