摘要
以油桃(Prunus persica var.necturina)‘中油5号’及其自交后代为试材,利用花柱S-RNase基因和花粉SFB基因的特异性引物进行PCR扩增,对扩增片段进行回收、克隆、测序和Blast分析,确定了‘中油5号’的自交不亲和基因型为S1S2(m)。序列分析结果显示,‘中油5号’桃S1-RNase和S2(m)-RNase与多个其他李属S-RNase基因氨基酸和核苷酸序列间都具有极高的相似性,SFB1和SFB2基因与多个其他李属SFB基因氨基酸和核苷酸序列间也都具有极高的相似性。进一步对‘中油5号’自交后代个体的S基因型进行检测,发现后代群体中存在3种S基因型,分别为S1S1、S1S2(m)和S2(m)S2(m),且分离比为8︰13︰7,与预期的比例1︰2︰1差异不显著(χ2=0.214<χ20.05,2=5.991),说明S-RNase基因和SFB基因都遵循两因素的孟德尔遗传规律进行连锁分离。此外,基因型为S1S1和S2(m)S2(m)的纯合体的存在,表明桃花粉SFB1和SFB2基因均不能被自身花柱S-RNase所识别,这是由于SFB1和SFB2基因翻译提前终止所造成的,从而使‘中油5号’表现出自交亲和性。
PCR were performed with specific primer sets of Prunus S-RNase and SFB alleles using ＇Zhongyou 5＇ （Prunus persica vat. necturina） as the template. The amplification products were extracted, cloned, sequenced and then Blast analysis, which were used to determine the S-genotype of this cultivar, S1S2（m）. Sequence analysis showed that Prunus persica S1-RNase and S2（m）-RNase alleles have exceptionally high identities with other Prunus S-RNase alleles, and, SFB1 and SFB2 alleles have exceptionally high identities with other Prunus SFB alleles, too. The S-genotypes of self-pollinated progeny of ＇Zhongyou 5＇ were detected, it is obviously that three classes were found in progeny, and the segregation ratio ofS1S1, S1S2（m） and S2（m）S2（m） was 8 ： 13 ： 7, which did fit to the expected ratio that was 2 1 ： 2 ： 1 （Z2 = 0.214 〈 Z o.o5,2 = 5.991 ） . This result showed that S-RNase and SFB alleles are tightly co-segregation according to the Mendel genetic model. Moreover, the existence of S1S1 and S2（m）S2（m） homozygotes suggested that SFB1 and SFB2 could not be cognized by selfS-RNase, due to that the proteinof these two SFB alleles were premature, which resulted in the ＇Zhongyou 5＇ is self-compatible.
出处
《园艺学报》
CAS
CSCD
北大核心
2012年第6期1035-1044,共10页
Acta Horticulturae Sinica
基金
江苏省自然科学基金项目(BK2011067)
