摘要
为鉴定8个中国梨品种的S基因型,使用梨自交不亲和基因(S-RNase)特异引物"FTQQYQ"和"anti-ⅡWP-NV",对8个梨品种的基因组DNA进行特异扩增,并对扩增片段进行回收、克隆、测序。使用生物信息学软件对各序列分析和经同源性搜索分析后,确定了各品种的S基因型。结果分别是:兰州花长把为S19S22,青面为S1S18,黄面为S1S12,早蜜为S19S29,大面黄为S1S19,无子黄为S28S16,大青皮为S34S19及金锤子为S16S19。
Using a pair of primers specific to pear S-RNases, PCR amplification from genomic DNAs of eight Chinese pear cuhivars were carried out. Then the PCR products were extracted, cloned and sequenced. The amplified fragments were assigned to their respective S-RNases by Blast analysis. The S-genotypes of the eight tested self-incompatible cuhivars were identified as follows： Lanzhou Huachangba （S19S22）, Qingmian （S1S18）, Huangmian （S1S12）, Zaomi （S19S29）, Damianhuang（S1S19）, Wuzihuang （S28S16）, Daqingpi （S34S19） and Jinchuizi （S16S19）.
出处
《果树学报》
CAS
CSCD
北大核心
2007年第6期757-760,共4页
Journal of Fruit Science
基金
国家林业局重点科学技术研究计划林业新技术开发与储备专项(2006-12)
湖南省科技三项基金(编号:00JZYZ115)
关键词
梨
自交不亲和性
S基因型
鉴定
Pyrus
Serf-incompatibility
S-genotype
Identification
