摘要
建立了直接竞争酶联免疫法(ELISA)测定金银花、枸杞、人参、板蓝根、大青叶和圆弧止痛片为代表的中药中速灭威的残留量.金银花等经过粉碎,加入甲醇提取、旋蒸、PBS复溶等简单前处理之后,再经过适度的稀释可以达到消除基质影响,用ELISA进行测定.中药样品添加回收率为64.00%～91.30%,变异系数均小于30.12%.结果表明,该方法可以简单、快速、灵敏、准确地检测出金银花、枸杞、人参、板蓝根、大青叶和圆弧止痛片等中药中速灭威的残留量.
A direct competitive ELISA was established in detecting Metolcarb residues in Chinese herbal medicines, such as Lonicera chrysantha, Lycium chinense, Radix Ginseng, Radix Isatidis, Folium Isatidis and Yuanhu painkillers. After Lo- nicera chrysantha and so on were mashed and homogenized, methanolextraction, rotary steaming, and PBS were used to make up to volume. Moderate dilution then could eliminate the influence of matrix, which was detected by ELISA. In Chi- nese herbal medicines, the recovery rate was 66%-91%, with variation coefficient less than 30%. The method is simple, quick, sensitive and accurate in detecting the Metolcarb residues in Chinese herbal medicines.
出处
《天津科技大学学报》
CAS
2012年第3期21-24,共4页
Journal of Tianjin University of Science & Technology
基金
国家科技重大专项课题(2009ZX09502-027)
关键词
ELISA
中药
速灭威
检测
ELISA
Chinese herbal medicines
Metolcarb
determination
