摘要
目的探讨AXL表达水平与上皮性卵巢癌细胞侵袭性的关系及其机制。方法使用OVCAR3细胞为研究载体,通过siRNA干扰细胞中AXL的表达,通过RT-PCR检测两组细胞中AXL以及MMP-9基因表达水平,Transwell法比较干扰组细胞与对照组细胞侵袭能力,并比较siRNA干扰组细胞与对照组细胞在裸鼠体内的成瘤能力。结果干扰组OVCAR3细胞中AXL以及基质金属蛋白酶-9表达水平明显降低,差异有统计学意义(P<0.05);实施siRNA转染组细胞侵袭细胞数量为73±10.5个,明显低于对照组261.5±21.7个,差异有统计学意义(P<0.05);干扰组细胞裸鼠成瘤的质量以及肿瘤细胞的数量均明显低于对照组,差异有统计学意义(P<0.05)。结论 AXL表达抑制时可能通过减少细胞内基质金属蛋白酶-9的mRNA的表达降低上皮性卵巢细胞的侵袭能力。
Objective To explore the effects and mechanisms of AXL on the invasive ability of epithelial ovarian carcinoma cell lines. Methods siRNA was used to inhibit the expression of AXL in OVCAR3, taken as the experiment group. The expression of MMP-9 and AXL were detected by RT-PCR, and the invasive ability of OVCAR3 was detected by Ttranswell method. The transplanted tumor number and weight were compared between the experiment group and control group. Results The expression level of AXL and MMP-9 were both significantly lower in the experiment group than in control group（P〈 0.05）. The invasive OVCAR3 number of the experiment group was 73 ± 10.5, which was significantly lower than that of control （261.5 ± 21.7） （P〈 0.05）. The transplanted tumor number and weight of the experiment group were significant lower than those of control group（P〈 0.05）. Conclusion The inhibition of expression of ALX could reduce the invasive ability of epithelial ovarian carcinoma by decreasing the mRNA expression of MMP-9.
出处
《肿瘤药学》
CAS
2012年第3期177-179,183,共4页
Anti-Tumor Pharmacy
基金
湖南省科技厅科技计划资助项目(编号:2010FJ3078)
