摘要
目的探讨乌司他丁对脂多糖(LPS)诱导的RAW264.7细胞一氧化氮(NO)释放和诱生型一氧化氮合酶(iNOSmRNA)表达的影响。方法应用LPS激活RAW264.7细胞株,与不同浓度组乌司他丁(100～10 000 U/ml)共同孵育,Griess试剂法测定NO释放量;实时荧光定量反转录聚合酶链反应(RT-PCR)技术分析iNOSmRNA的表达。结果高浓度的乌司他丁(1 000～10 000 U/ml)能抑制LPS诱导的RAW264.7细胞NO的表达(P<0.05),下调iNOSmRNA含量(P<0.05);低浓度乌司他丁(100 U/ml)对LPS诱导的RAW264.7细胞NO、iNOSmRNA表达与LPS单独处理组比较,差异无统计学意义(P>0.05)。结论高浓度乌司他丁抑制LPS诱导的巨噬细胞NO释放和iNOSmRNA表达,这种抑制与浓度相关。
Objective To investigate the effect of ulinastatin on the lipopolysaccharide （LPS）-induced production of nitric oxide （NO） and the mRNA expression of inducible nitric oxide synthase （iNOS） in macrophages. Methods The RAW264.7 macrophage cells activated with LPS were pretreated with ulinastatin of different concentrations （100 - 10 000 U/ml）. The production of NO was determined by the Griess test. The mRNA expression of iNOS was determined by Real-time RT-PCR. Results Ulinastatin,especially in high concentration ranges（ 1 000 - 10 000 U/ml）, inhibited LPS-induced production of NO （P 〈 0.05 ）, decreased the mRNAs expression of iNOS （P 〈 0.05 ） ;There was no significant difference in NO and the mRNA expression of iNOS in LPS-induced RAW 264.7 cells between ulinastatin of low concentration（ 100 U/ml） and LPS alone （P 〉 0.05 ）. Conclusion High concentration ulinastatin can inhibit the production of NO and mRNA expression of iNOS in LPS-induced macrophages, and the effect is correlated with the concentration of ulinastatin.
出处
《广西医学》
CAS
2012年第5期529-530,539,共3页
Guangxi Medical Journal
基金
广西自然科学基金(桂科自0832147)
广西医药卫生科研课题(桂卫重200713)
关键词
乌司他丁
巨噬细胞
一氧化氮
诱生型一氧化氮合酶
脂多糖
Ulinastatin
Macrophages
Nitric oxide
Inducible nitric oxide synthase
Lipopolysaccharide
