摘要
目的:建立一种快速、简便、特异性高的鸭瘟病毒(DPV)环介导等温扩增(LAMP)检测方法。方法:根据Gen Bank中DPVUI6基因的保守序列设计一套特异性引物,并对反应条件进行优化,建立DPV的LAMP可视化检测方法。结果:建立的LAMP方法对其他鸭常见病原体无扩增反应;可通过肉眼观察颜色直接判定结果;敏感性可达0.1fg,是常规PCR方法的100倍;扩增反应只须在常规水浴锅中进行,可在1 h内完成。结论:建立的DPV LAMP方法简便、快速、灵敏、特异,可用于DPV感染的快速检测。
Objective： To develop a simple, rapid, specific loop-mediated isothermal amplification（LAMP） assay for the detection of duck plague virus（DPV）. Methods： According to the sequences of DHV UL6 gene in Gen- Bank, a set of primers were designed, and the reaction conditions were optimized. Results： The results showed that the LAMP assay was no cross-reactivity with the other pathogen of duck, and the presence of DPV could be detected by naked eyes. The detection limit of this LAMP method was 0.1 fg, which was 100 fold higher than the routine PCR. The amplification could be finished within 1 h. Conclusion： These results suggest that this LAMP assay is a simple, rapid, specific method for the detection of DPV in the field.
出处
《生物技术通讯》
CAS
2012年第3期426-429,共4页
Letters in Biotechnology
基金
广西科技项目(桂科攻10100014-5和桂科专项11-3)
广西特聘专家专项
国家百千万人才工程人选专项(945200603)
关键词
鸭瘟病毒
环介导等温扩增
检测
duck plague virus
loop-mediated isothermal amplification
detection
