摘要
目的探讨TCF7L2基因rs290487多态性与辽宁地区2型糖尿病发病的相关性。方法选取2008年12月～2010年7月在中国医科大学附属第四医院内分泌科临床确诊为2型糖尿病的患者255例,提取患者全血基因组DNA,PCR扩增TCF7L2基因单核苷酸rs290487,PCR产物经ACCⅡ酶切后,琼脂糖凝胶电泳分析rs290487的多态性及等位基因rs290487的突变与2型糖尿病发病的相关性。结果 TCF7L2基因rs290487 TT、TC、CC 3个基因型在2型糖尿病组与对照组的分布频率分别为48.24%、46.67%、5.10%和50.74%、40.39%、8.87%,组间差异均无统计学意义(P>0.05);TCF7L2基因rs290487多态位点在两组间的基因型分布符合Hardy-Weinberg平衡定律;Binary Logistic回归校正了性别、年龄、体重指数、血压、血脂等因素后,TCF7L2基因rs290487多态性与2型糖尿病的发生无相关性(P>0.05)。结论辽宁地区人群中TCF7L2基因rs290487多态性与2型糖尿病的发生无相关性。
Objective To investigate the relationship between TCFTL2 gene rs290487 polymorphisms and type 2 diabetes in Liaoning Area, China. Methods Genomic DNAs were extracted from blood of 255 patients with type 2 diabetes confirmed in Department of Endocrinology, The Fourth Affiliated Hospital of China Medical University during December 2008 to July 2010, with which the single nucleotide rs290487 of TCFTL2 gene was amplified by PCR. The PCR product was digested with ACC 11 and analyzed for polymorphisms by agarose gel electrophoresis, based on which the relationships of polymorphisms and allelic mutation of rs290487 to onset of type 2 diabetes were analyzed. Results The distribution frequencies of genotypes TT, TC and CC of TCF7L2 gene rs290487 in patients with type 2 diabetes were 48. 24%, 46. 67% and 5. 10%, while those in healthy persons as control were 50. 74%, 40. 39% and 8. 87%, respectively, which showed no significant difference （P 〉 0. 05）. The genotype distribution of TCF7L2 gene rs290487 polymorphic site in the two groups met Hardy-Weinberg law of equilibrium. After sex, age, bodyweight index, blood pressure and blood fat were corrected by Binary Logistic regression, no relationship was observed between TCF7L2 gene rs290487 polymorphisms and type 2 diabetes （P 〉 0. 05）. Conclusion TCFTL2 gene rs290487 polymorphisms showed no relationship to type 2 diabetes in populations in Liaoning Area.
出处
《中国生物制品学杂志》
CAS
CSCD
2012年第6期740-742,753,共4页
Chinese Journal of Biologicals
基金
辽宁省教育厅科学技术研究项目(2009A772)
关键词
糖尿病
2型
基因
多态性
单核苷酸
Diabetes, type 2
Gene
Polymorphisms, single nucleotide
