摘要
目的:观察1次大强度运动后骨骼肌泛素蛋白酶体途径组成成分基因表达的变化,探讨该途径在运动后骨骼肌蛋白质降解中的作用。方法:36只雄性SD大鼠,体重190～220 g,跑台运动适应3天,休息1天,取6只为安静对照,其余大鼠进行1次1小时跑台运动(25 m/min,5%坡度)。运动0.5小时即刻、运动1小时即刻、运动后1小时、运动后2小时、运动后6小时,分别选6只大鼠取腓肠肌,用Real-Time PCR法测定泛素、MuRF1、MAFbx mRNA含量,用Western Blot法检测泛素化蛋白含量,用高效液相色谱法检测肌肉3-MH含量。结果:运动0.5、1小时即刻至运动后6小时,腓肠肌泛素mRNA含量与安静时比较无显著性差异(P>0.05)。1小时运动后1、2和6小时,腓肠肌MAFbx和MuRF1 mRNA含量较安静时显著升高(P<0.01,P<0.05)。1小时运动后6小时,腓肠肌泛素化蛋白含量显著高于安静时(P<0.01)。运动0.5小时、1小时即刻和运动后6小时,腓肠肌3-MH含量显著高于安静(P<0.01)。结论:1次大强度运动后,骨骼肌泛素蛋白酶体途径基因表达升高,骨骼肌收缩蛋白降解增加。
Objective To study the role of ubiquitin （UB）-proteasome pathway （UPP） of skeletal muscle on the protein degradation after an acute exercise. Methods Male SD rats （n=36） were adapted to treadmill exercise for 3 days. After 1-day rest,6 rats were used as quiet control group,and the re- maining rats ran on the treadmill for 1 hour （25m/min,5% slope）. Every 6 rats were killed at 0.5 h and 1 h immediate after exercise,and at 1 h, 2 h,6 h after exercise,and the changes in the genetic expres- sion of Ub,MuRF1 ,MAFbx mRNA ,ubiquitin-conjugated protein level and 3-MH were assayed with Re- al-Time PCR, Western Blot, and high-efficiency liquid chromatography, respectively. Results （1）There was no significant difference in the content of Ub mRNA before and after exercise. （2）Contents of MAF- bx and MuRF1 mRNA were higher after lhr exercise than at rest（P 〈 0.01 ,P 〈 0.05,respectively）. （3）Ubiquitinated protein level was higher at 6 h after 1 h exercise than at rest（P 〈 0.05）. （4）3-MH con- tent was higher 0.5 h and 1 h immediately after exercise and at 6h post-exercise than at rest. Conclusion Both genetic expressions of ubiquitin-proteasome pathway and degree of contractile protein degra- dation of skeletal muscle were enhanced after one ut of high-intensity exercise .
出处
《中国运动医学杂志》
CAS
CSCD
北大核心
2012年第5期414-419,426,共7页
Chinese Journal of Sports Medicine
基金
国家自然科学基金(30971411)
浙江省自然科学基金(LY12C11002)资助
关键词
大强度运动
骨骼肌
泛素蛋白酶体途径
蛋白质降解
acute exercise, skeletal muscle, ubiquitin-proteasome pathway,protein degradation
