摘要
研究目的运用蛋白质组学的理论和方法研究急性大强度力竭运动大鼠骨骼肌蛋白质表达特征。方法10只雄性SD大鼠随机按体重配对分为安静组(5只)和运动组(5只)。运动组大鼠按Bedford大强度运动模型运动至力竭,力竭后3h和安静组大鼠同时断头处死,提取后肢腓肠肌全蛋白质做双向凝胶电泳。结果用bio-radPDquest软件对电泳图谱进行分析,安静组和运动组各获得667±35和572±28个蛋白质点,在运动组中出现新增和缺失的蛋白质点,同时还出现蛋白质表达上调和下调的蛋白质点。对表达差异的蛋白质点做MALDI-TOF-TOF质谱分析,鉴定出7个运动后表达差异的蛋白质。结论大鼠大强度力竭运动后蛋白质发生了质和量的变化,为更加深入地研究运动性疲劳打下了良好的基础。
The purpose of this study was to analyze the differential expression of SD rat＇s skeletal muscle proteome after acute exhaustive exercise. Ten male SD rats were randomly divided into exercise and rest group according to their weight. The exercise group was sent to a one time intensive exhausted training treadmill and were executed by decollation third hour after exhaustive exercise. The rest group was the same doing like exercise group. The lower limb gastrocnemius muscle protein waa extracted and subsequently separated by 2D electrophoresis. The result showed that 572±28 protein spots were found in the exercise group and 667 ± 35 in the rest group. It concluded that there had been qualitative and quantitative change in protein after high intensity and exhaustive training, and it laid solid foundation for further research on exercise fatigue.
出处
《体育科学》
CSSCI
北大核心
2006年第5期59-63,68,共6页
China Sport Science
