摘要
目的:建立固相萃取-反相高效液相色谱法测定植物油中苯并(a)芘的检测方法。方法:植物油中的苯并(a)芘经正己烷超声提取后,用中性氧化铝柱净化,采用ZORBAX SB–Phenyl色谱柱(4.6×250 mm,5μm)分离,以乙腈-水为流动相,荧光检测器检测,激发波长384 nm,发射波长406 nm。结果:方法回收率为87.2%～97.0%,标准偏差(RSD)为2.48%。结论:植物油中苯并(a)芘经中性氧化铝柱净化后,杂质干扰明显减少,采用反相高效液相色谱法、荧光检测器对苯并(a)芘进行分离检测,灵敏度较高,分离度良好、回收率满意,适于植物油中苯并(a)芘的测定。
Objective：To establish a solid phase extraction-reversed phase high performance liquid chromatographic method for determination of benzo（a）pyrene in vegetable oil.Methods： Benzo（a）pyrene in sample was firstly extracted with hexane,purified with neutral Al2O3 SPE Column and determined with HPLC.The column used was ZORBAX SB–Phenyl column（4.6×250 mm,5 μm）,mobile phase was acetonitrile-water and FLD was adopted with excitation and emission wavelengths at 384 nm and 406 nm,respectively.Results： The recoveries of the method were 87.2%~97.0%,with the detection limit of 0.1 μg/kg and RSD% of 2.48%.Conclusion： The method is sensitive with satisfied recoveries.The method is suitable for determination of benzo（a）pyrene in vegetable oil.
出处
《中国卫生检验杂志》
北大核心
2012年第5期990-992,共3页
Chinese Journal of Health Laboratory Technology
