摘要
在29℃和160 r/min条件下,以广东云浮矿山酸性废水为种源,采用9K培养基,经过富集培养得到氧化亚铁硫杆菌A.f.a。利用分批摇床实验,研究了在A.f.a作用下,溶液初始NH4+-N、PO34--P、Mg2+、Ca2+和Cu2+等基本营养成分对Fe2+生物氧化的影响。结果显示,随着NH4+-N、PO34--P、Ca2+或Cu2+离子浓度增加,Fe2+生物氧化率都先增大后降低,各离子最佳浓度分别为84.8、35.6、2.4和254.5 mg/L;Fe2+生物氧化率随着Mg2+浓度的增加逐渐增大,并趋于稳定,其限制浓度为88.8 mg/L。上述结果可用于控制营养条件促进Fe2+的生物氧化。
A strain of Acidithiobacillus ferrooxidans（A.f.a） which was from acid mine drainage of Yunfu mine in Guangdong,was obtained by enrichment cultivation using 9K medium,on a rotary shaker at 160 r/min and a constant temperature of 29℃.The effects of nutrients such as NH＋4-N,PO3-4-P,Mg2＋,Ca2＋ and Cu2＋ on ferrous biooxidation by the strain A.f.a were studied with batch experiments.The results show that the ferrous biooxidation rate is tended to increase first and then decrease with increasing NH＋4-N,PO3-4-P,Ca2＋ or Cu2＋ concentration.Their optimum concentrations are 84.8,35.6,2.4 and 254.5 mg/L,respectively.With increasing Mg2＋ concentration,the ferrous biooxidation rate increases nearly to stability,and the limiting concentration is 88.8 mg/L.The results can be used to improve ferrous biooxidation by controlling the conditions of nutrients.
出处
《环境工程学报》
CAS
CSCD
北大核心
2012年第6期1901-1906,共6页
Chinese Journal of Environmental Engineering
基金
南京大学环境学院污染控制与资源化研究国家重点实验室开放基金
国家自然科学基金资助项目(50978133)
关键词
营养成分
硫酸亚铁
氧化亚铁硫杆菌
生物氧化
nutrients
ferrous sulphate
Acidithiobacillus ferrooxidans
biooxidation
