摘要
目的研究肠康片的定性定量方法。方法采用薄层色谱法对肠康片中木香进行定性鉴别,采用高效液相色谱法对肠康片中吴茱萸进行定性鉴别,并采用高效液相色谱法对盐酸小檗碱进行含量测定。结果薄层色谱分离清晰,阴性样品无干扰。运用高效液相色谱法能检出吴茱萸中所含的吴茱萸碱和吴茱萸次碱,阴性样品无干扰。盐酸小檗碱在0.038 76～0.969 2μg线性良好,r=0.999 8,平均回收率为101.9%,RSD=1.6%(n=6)。结论本方法专属性强,灵敏度高,重现性好,能更好地对肠康片进行质量控制。
Objective To establish a qualitative and quantitative method for Changkang Pian. Methods Radix Auck landiae was identified by TLC, Fructus Evodiae （prepared） was identified by HPI.C, and berberine hydrochloride was determined by HPLC. Results The TLC points developed were fairly clear and the negative sample showed no interference. Evodiamine and rutaecarpine were identified by HPLC and negative sample showed no interference. Berberine hydrochloride was linear at 0. 038 76-0. 969 2 μg （r=0. 999 8）, the and average recovery was 101.9%, RSD= 1.6% （n=6） . Conclusion The method is selective, sensitive and reproductive, which can be used for the quality control of Changkang Pian.
出处
《中南药学》
CAS
2012年第4期312-314,共3页
Central South Pharmacy
