摘要
目的建立定心丸质量标准。方法采用薄层色谱法对定心丸中的甘草,当归,远志进行定性鉴别,并用高效液相色谱法对定心丸中黄芩苷的含量进行测定,色谱柱为C18柱(250mm×4.6mm,5μm),流速为1.0mL·min-1,流动相为乙腈-0.2%磷酸(28∶72),检测波长为280nm。结果甘草,当归,远志薄层色谱分离良好,斑点清晰可见;黄芩苷含量在0.1934～1.1606μg内线性关系良好,平均加样回收率为101.45%(RSD=1.2%,n=6),精密度RSD=1.4%。结论该方法结果准确、重复性好,可作为该制剂的质量控制方法。
OBJECTIVE To establish the quality standard for Dingxin pills. METHODS TLC was applied to identify Radix Glycyrrhizae,Radix Angelica Sinensis and Radix Polygalae Willd in this prescription, and baicalin content in Radix Scutellariae was determined by HPLC. A C18 column（250 mm ×4.6 mm,5 μm） was used with the mobile phase of acetonitrile-0.2% phosphoric acid （22：78 ） at the detection wavelength of 280 nm. RESULTS Radix Glycyrrhizae ,Radix Angelica Sinensis and Radix Polygalae could be identified by TLC. The baicalin linear rang of 0. 193 4 - 1. 160 6μg, and the average recovery was 101.45% （ RSD = 1.2% ,n = 6）. CONCLUSION The evaluation methods are simple, sensitive, accurate and reproducible, and can be used for the quality control of this medicine.
出处
《中国现代应用药学》
CAS
CSCD
北大核心
2009年第3期237-240,共4页
Chinese Journal of Modern Applied Pharmacy
关键词
甘草
当归
远志
黄芩苷
薄层色谱法
高效液相色谱法
Radix Et Rhicoma Glycyrrhizae
Radix Angelica Sinensis
Radix Polygalae
baicalin
TLC
HPLC
