摘要
磷饥饿条件下番茄幼苗的Ｈ~＋分泌速率明显提高。质膜质子泵专一性抑制剂钒酸盐能显 著抑制番茄幼苗的 Ｈ~＋分泌，也能显著抑制其Ｐｉ吸收。此结果表明，磷饥饿时番茄幼苗 Ｐｉ吸收 速率的变化与 Ｈ~＋分泌速率的变化之间可能具有一定的相关性，并进一步暗示质膜 Ｈ~＋－ＡＴＰａｓｅ可能 参与其中。本文结果还表明，Ｐｉ／Ｈ~＋的准量关系约为１∶１。
Changes of H^+ extrusion rates and the relationship between H^+ extrusion and Pi uptake of tomato seedlings during phosphate starvation were studied. The results revealed that phosphate starvation increased the H^+ extrusion rates of tomato seedlings and it also indicated that vanadate, a specific inhibitor of PM H^+-ATPase, strongly inhibited not only Pi uptake but also H^+ extrusion of tomato seedlings, which were exhibited more strongly in Pi starved conditions than in the control. These results indicated that the changes of Pi uptake rates and H^+ extrusion rates may be related, and furthermore, the PM H^+-ATPase may be involved in these changes during phosphate starvation. The results also revealed that the quasi-ratio of Pi/H^+ was about 1∶1.
基金
国家自然科学基金
关键词
番茄
磷饥饿
H^+分泌
H^+泵
磷吸收
tomato (Lycopersicon esculentum
phosphate starvation
phosphorus uptake
H^+ extrusion, plasmalemma (PM) H^+-ATPase
quasi-ratio of Pi/H^+
