摘要
目的:采用气相色谱法拆分醒脑静注射液中龙脑对映体,并通过对映体的分离测定含量。方法:采用CYCLOSIL-B(30m×0.25 mm×0.25μm)手性毛细管色谱柱、FID检测器,以内标法测定醒脑静注射液中左旋龙脑和右旋龙脑的含量。结果:龙脑对映体在CYCLOSIL-B手性毛细管色谱柱上具有较好的分离效果,其分离度为2.04。左旋龙脑和右旋龙脑线性范围分别为0.048～0.960和0.050～1.008 mg·mL-1(r≥0.9991);冰片投料为天然冰片的样品中右旋龙脑平均回收率(n=6)为98.4%,RSD为1.8%;冰片投料为艾片的样品中左旋龙脑的平均回收率(n=6)为99.1%,RSD为1.9%。结论:所建立的方法简单、准确、可靠,能用于醒脑静注射液的质量控制。
Objective：To develop a GC method to separate and determine the enantiomers of borneol.Methods：The enantiomers of borneol were separated by CYCLOSIL-B chiral capillary chromatographic column（30 m×0.25 mm×0.25 μm）and determined by internal standard method,with FID as detector.Results：The enantiomers of borneol could be baseline separated at CYCLOSIL-B chiral capillary chromatographic column（30 m×0.25 mm×0.25 μm）,Resolution of the enantiomers of borneol was 2.04.The calibration curves were linear in the ranges of 0.048-0.960 mg·mL-1 for（L）-borneol and 0.050-1.008 mg·mL-1 for（R）-borneol.For sample which came from Borneolum,the average recovery（n=6）of（R）-borneol was 98.4% with RSD of 1.8%,respectively;For sample which came from L-Borneolum,the average recovery（n=6）of（L）-borneol was 99.1% with RSD of 1.9%.Conclusion：This method is simple,rapid and accurate with good reproducibility,and suitable for the quality control of Xingnaojing injection.
出处
《药物分析杂志》
CAS
CSCD
北大核心
2012年第5期873-876,共4页
Chinese Journal of Pharmaceutical Analysis
