摘要
目的探讨不同剂量的川芎嗪对高糖透析液作用下慢性大鼠腹膜透析（腹透）模型腹膜间皮细胞的形态和功能的影响及它们之间的关系。方法４０只ＳＤ大鼠随机分为４．２５％腹透液（ＨＧ组）、４． ２５％腹透液＋４０ｍｇ／Ｌ川芎嗪（ＨＧＬ组）、４． ２５％腹透液＋ １６０ｍｇ／Ｌ川芎嗪（ＨＧＨ组）、对照组。除对照组外，余 ３组每天分别腹腔内注入 ２０ ｍｌ含不同剂量川芎嗪的 ４． ２５％透析液［０（ＨＧ）、４０ｍｇ／Ｌ（ＨＧＬ）、１６０ｍｇ／Ｌ（ＨＧＨ）］。８周后进行腹膜功能试验，同时对细胞印片进行形态学分析。结果与ＨＧＨ和ＨＧ组相比，ＨＧＬ组腹腔内液体量和净超滤量显著增高（Ｐ＜０．０１），透出液与血浆尿素浓度比（Ｄ／Ｐｕｒｅａ）显著降低，４ ｈ透出液／透析液葡萄糖（Ｄ／Ｄ０）值和尿素清除率（Ｃｕｒｅａ）显著增高。但总重吸收率和直接淋巴吸收率在各透析组之间无显著差异。细胞印片上间皮细胞的细胞密度在ＨＧＬ组比ＨＧＨ组和ＨＧ组显著增加，而表面积显著减少。低剂量川穹嗪显著提高腹膜超滤量与间皮细胞表面积减少密切相关（ｒ＝－０．９４４，Ｐ＜０．０５）。结论低剂量川芎嗪加入高糖透析液中长期应用可显著提高腹膜超滤量。
Objective To investigate the influence of different concentrations of ligustrazine on the cell morphology and the peritoneal membrane function and their relationship in chronic peritoneal dialysis rats with infusion of high glucose dialysate. Methods 40 SD rats were divided randomly into 4 groups. Except control group, other groups daily received infusion of 20 ml 4. 25% glucose dialysate containing different concentrations of ligustrazine [0 (HG), 40 mg/L (HGL), 160 mg/L (HGH) ] respectively for 8 weeks. The peritoneal membrane function was investigated by peritoneal transport test, and the rat peritoneal mesothelial cells(PMCs )morphology was analyzed from the cell imprints. Results Compared to the HG and HGH groups, the intraperitoneal volume and net ultrafiltration in HGL group were significantly higher (P < 0.01 ), D/Purea significantly lower, and D/Do and Curea after 4 hours of dialysis significantly higher. The peritoneal fluid absorption rate as well as direct lymphatic absorption rate had no significant difference between all the groups. The density of cell population from analysis of cell imprints in HGL group was significantly higher than that of HGH and HG groups, but the mean surface area was significantly lower. hong-term use of low dose ligustrazine in the high glucose dialysate could significantly increase the ultrafiltration volume, which was significantly correlated to the decreasing of cell surface area (r = - 0. 944, P < 0. 05) of PMCs. Conclusion Low-dose ligustrazine added to high glucose dialysate can significantly increase the ultrafiltration volume, which might related to the decrease of the hypertrophy of PMCs.
出处
《中华肾脏病杂志》
CAS
CSCD
北大核心
2000年第3期143-146,共4页
Chinese Journal of Nephrology
基金
卫生部临床重点攻关资助项目!(97040228)
关键词
腹膜透析
川芎嗪
透析效能
腹膜间皮细胞
Peritoneal dialysis
Ligustrazine
Adequacy of dialysis
Peritoneal mesothelial cells
