摘要
目的:观察丙泊酚对大鼠单肺通气时肺代谢酶的影响,为丙泊酚在胸科麻醉中的应用提供实验研究的数据。方法:40只健康清洁级SD大鼠随机分成四组:P1组:空白组(灌注液中无丙泊酚);P2组:含36mg/L丙泊酚;P3组:含24mg/L丙泊酚;P4组:含12mg/L丙泊酚,每组10只。按随机顺序取大鼠进行实验,称量体重,麻醉,气管切开插管接呼吸机行单肺机械通气1h。然后取出肺组织。-70℃保存,以备统一作Ca2+-ATPase、Na+-k+ATPase测定。结果:肺组织Na+-K+ATPase活力:P1组通气侧活力显著低于P2、P3、P4组通气侧(P<0.01);P1组非通气侧活力显著低于P2、P3、P4组非通气侧(P<0.01)。各组非通气侧均低于同组通气侧(P<0.01或0.05)。肺组织Ca2+-ATPase活力:P1组通气侧显著低于P2、P3、P4组通气侧(P<0.01);P1组非通气侧显著低于P2、P3、P4组非通气侧(P<0.01)。各组非通气侧均显著低于同组通气侧(P<0.01)。结论:通过对大鼠单肺通气时的灌注实验研究发现,丙泊酚对单肺通气时的肺代谢酶有保护作用,其程度与丙泊酚浓度在一定范围内无关。
Objective：To investigate the effect of propofol on metabolic enzyme of rats during one-lung ventilation.Methods：40 rats randomly divided into 4 groups（n=10, each）：group P1 （no propofol in the perfusate ）, group P2 （the concentration of propofol in perfusate was 36mg/L）, group P3（24 nag/L）, group P4 （12 rag/L）. Metabolic enzyme was quantified after one-lung ventilation .After OLV finished , lung tissue sample were harvest for lhe criterions of the Na^-K＋ATPase activity, CaE＋ATPase activity.Results：（1）The Na＋-K＋ATPase activity： as compared to the values in group P2, group P3 and group P4 ventilated lung, the Na＋-K＋ATPase activity was low in group PI ventilated lung（P〈0.01） ; as compared to the values in group P2, group P3 and group P4airproof lung, the Na＋-K＋ATPase activity was low ill group PI airproof lung（P〈0.01） ; as compared to the values in reciprocal group ventilated lung, the Na^-K＋ATPase activity was low in each group airproof lung（P〈0.01 or 0.05）.（2）The Ca2＋-ATPase activity： as compared to the values in group P2, group P3 and group P4 ventilated lung, the Ca2＋-ATPase activity was low in group Pt ventilated lung（P〈0.01） ; as compared to the values in group P2 , group P3 and group P4 airproof lung, the Ca2＋-ATPase activity was low in group Pl airproof lung（P〈0.01） ; as compared to the values in reciprocal group ventilated lung, the Ca2＋-ATPase activity was low in each group airproof lung（P〈0.01）.Conclusion：These results indicate that the propofol show the protective effects on lung metabolic enzyme of rats during one-lung ventilation , and the effects do not seem to correlate with the concentration of propofol.
出处
《中国医学创新》
CAS
2012年第13期6-7,共2页
Medical Innovation of China
